Three dimensional (3D) histology in daily routine: practical implementation and its evaluation. (29th October 2014)

• Record Type: Journal Article
• Authors: Carola Eberle, Franziska; Kanyildiz, Mukaddes; Maria Schnabl, Saskia; Schulz, Claudia; Häfner, Hans‐Martin; Adam, Patrick; Breuninger, Helmut
• Is Part Of: Journal der Deutschen Dermatologischen Gesellschaft. Volume 12:Number 11(2014)
• Page Start: 1028
• Page End: 1035
• Publication Date: 2014-10-29
• Subjects: Skin -- Diseases -- Periodicals; Dermatology -- Periodicals; 616.5005
• DOI: 10.1111/ddg.12466
• Languages: English
• ISSNs: 1610-0379

Abstract

<abstract abstract-type="main"> <title>Summary</title> <sec id="ddg12466-sec-0010" sec-type="section"> <title>Background</title> <p>The continuous evaluation of the edges of a tumor by means of three‐dimensional (3D) histology often appears complicated and require the surgeon and dermatopathologist to work together closely. We present clear rules that allow communication between all parties involved and then verify their application in daily routine.</p> </sec> <sec id="ddg12466-sec-0020" sec-type="section"> <title>Methods</title> <p>Tissue processing, interpretation of results, as well as communication between the surgeon and the dermatopathologist are based on an algorithm with the aid of exact times and embedding cassettes, which allow precise topographic orientation. We evaluated the use of this method in daily clinic practice, taking into account 947 operated basal cell carcinomas in regard to the development of recurrent tumors.</p> </sec> <sec id="ddg12466-sec-0030" sec-type="section"> <title>Results</title> <p>At a median follow‐up of 47 months, 10 of the 947 operated basal cell carcinomas (1.1 %) recurred. Sclerodermiform basal cell carcinomas and basal cell carcinomas which could not be curatively resected (R0 resection) during the initial surgery showed a significantly higher recurrence rate (p < 0.05 and p < 0.001).</p> </sec> <sec id="ddg12466-sec-0040" sec-type="section"> <title>Conclusions</title> <p>Standardized rules for dealing with excised tissue allow an effective application of 3D histology in daily clinical practice. 3D histology results in low recurrence rates. Sclerodermiform basal cell carcinomas which could not be curatively resected (R0 resection) were identified as a risk group for the development of recurrent tumors.</p> </sec> </abstract>