Imatinib Activates Pathological Hypertrophy by Altering Myocyte Calcium Regulation. Issue 5 (16th June 2014)

• Record Type: Journal Article
• Authors: Barr, Larry A.; Makarewich, Catherine A.; Berretta, Remus M.; Gao, Hui; Troupes, Constantine D.; Woitek, Felix; Recchia, Fabio; Kubo, Hajime; Force, Thomas; Houser, Steven R.
• Is Part Of: Clinical and translational science. Volume 7:Issue 5(2014)
• Page Start: 360
• Page End: 367
• Publication Date: 2014-06-16
• Subjects: Medicine, Experimental -- Periodicals; Medical innovations -- Periodicals; 616.027
• DOI: 10.1111/cts.12173
• Languages: English
• ISSNs: 1752-8054

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="cts12173-sec-0010" sec-type="section"> <title>Background</title> <p>Imatinib mesylate is a selective tyrosine‐kinase inhibitor used in the treatment of multiple cancers, most notably chronic myelogenous leukemia. There is evidence that imatinib can induce cardiotoxicity in cancer patients. Our hypothesis is that imatinib alters calcium regulatory mechanisms and can contribute to development of pathological cardiac hypertrophy.</p> </sec> <sec id="cts12173-sec-0020" sec-type="section"> <title>Methods and Results</title> <p>Neonatal rat ventricular myocytes (NRVMs) were treated with clinical doses (low: 2 μM; high: 5 μM) of imatinib and assessed for molecular changes. Imatinib increased peak systolic Ca<sup>2+</sup> and Ca<sup>2+</sup> transient decay rates and Western analysis revealed significant increases in phosphorylation of phospholamban (Thr‐17) and the ryanodine receptor (Ser‐2814), signifying activation of calcium/calmodulin‐dependent kinase II (CaMKII). Imatinib significantly increased NRVM volume as assessed by Coulter counter, myocyte surface area, and atrial natriuretic peptide abundance seen by Western. Imatinib induced cell death, but did not activate the classical apoptotic program as assessed by caspase‐3 cleavage, indicating a necrotic mechanism of death in myocytes. We expressed AdNFATc3‐green fluorescent protein in NRVMs and showed imatinib treatment significantly increased nuclear factor of activated T cells translocation that was inhibited by the calcineurin inhibitor FK506 or CaMKII inhibitors.</p> </sec> <sec id="cts12173-sec-0030" sec-type="section"> <title>Conclusion</title> <p>These data show that imatinib can activate pathological hypertrophic signaling pathways by altering intracellular Ca<sup>2+</sup> dynamics. This is likely a contributing mechanism for the adverse cardiac effects of imatinib.</p> </sec> </abstract>