Preliminary characterisation of nanotubes connecting T‐cells and their use by HIV‐1. (16th September 2014)

• Record Type: Journal Article
• Authors: Lachambre, Simon; Chopard, Christophe; Beaumelle, Bruno
• Is Part Of: Biology of the cell. Volume 106:Number 11(2014:Nov.)
• Page Start: 394
• Page End: 404
• Publication Date: 2014-09-16
• Subjects: Cytology -- Periodicals; Electron microscopy -- Periodicals; 571.6
• DOI: 10.1111/boc.201400037
• Languages: English
• ISSNs: 0248-4900

Abstract

<abstract abstract-type="graphical" xml:lang="en" id="boc201400037-abs-0001"> <title>Research article</title> <p>We characterised tunnelling nanotubes (TNTs) connecting T‐cells. We found that their tip is enriched in actin regulators such as PI(4, 5)P₂, ezrin and Exo70. HIV‐1 Gag is also recruited by PI(4, 5)P₂ and accumulated at the TNT apex that seems to be a preferential virus assembly platform for budding in close proximity with the connected cell to enable efficient virus transmission. Tetraspanins, especially CD81, were enriched in TNTs but did not show any specific accumulation at their tip. <boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgh23dt9rs8" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>