Effects of Pb(Ⅱ) exposure on Chlorella protothecoides and Chlorella vulgaris growth, malondialdehyde, and photosynthesis‐related gene transcription. Issue 11 (24th April 2013)

• Record Type: Journal Article
• Authors: Xiong, Bang; Zhang, Wei; Chen, Lin; Lin, Kuang‐Fei; Guo, Mei‐Jin; Wang, Wei‐Liang; Cui, Xin‐Hong; Bi, Hua‐Song; Wang, Bin
• Is Part Of: Environmental toxicology. Volume 29:Issue 11(2014:Nov.)
• Page Start: 1346
• Page End: 1354
• Publication Date: 2013-04-24
• Subjects: Water quality bioassay -- Periodicals; Water -- Pollution -- Toxicology -- Periodicals; Microbiological assay -- Periodicals; Toxicity testing -- Periodicals; Environmental toxicology -- Periodicals; Environmental Pollution -- Periodicals; Environmental Pollutants -- Periodicals; Environmental Monitoring -- Periodicals; Écotoxicologie -- Périodiques; Pollution -- Périodiques; 615.902
• DOI: 10.1002/tox.21865
• Languages: English
• ISSNs: 1520-4081

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>Greater exposure to Pb(Ⅱ) increases the likelihood of harmful effects in the environment. In this study, the aquatic unicellular alga <italic>Chlorella protothecoides</italic> (<italic>C. protothecoides</italic>) and <italic>Chlorella vulgaris</italic> (<italic>C. vulgaris</italic>) were chosen to assess the acute and chronic toxicity of Pb(Ⅱ) exposure. Results of the observations show dose‐response relationships could be clearly observed between Pb(Ⅱ) concentration and percentage inhibition (PI). Exposure to Pb(Ⅱ) increased malondialdehyde (MDA) content by up to 4.22 times compared with the control, suggesting that there was some oxidative damage. ANOVA analysis shows that Pb(Ⅱ) decreased chlorophyll (chl) content, indicating marked concentration‐dependent relationships, and the lowest levels of chl a, chl b, and total‐chl were 14.53, 18.80, and 17.95% of the controls, respectively. A real‐time PCR assay suggests the changes in transcript abundances of three photosynthetic‐related genes. After 120 h exposure Pb(Ⅱ) reduced the transcript abundance of <italic>rbc</italic>L, <italic>psa</italic>B, and <italic>psb</italic>C, and the relative abundances of the three genes of <italic>C. protothecoides</italic> and <italic>C. vulgaris</italic> in response to Pb(Ⅱ) were 54.66–98.59, 51.68–95.59, 37.89–95.48, 36.04–94.94, 41.19–91.20, and 58.75–96.80% of those of the controls, respectively. As for 28 d treatments, the three genes displayed similar inhibitory trend. This research provides a basic understanding of Pb(Ⅱ) toxicity to aquatic organisms. © 2013 Wiley Periodicals, Inc. Environ Toxicol 29: 1346–1354, 2014.</p> </abstract>