Involvement of Histamine in Endothelium‐Dependent Relaxation of Mesenteric Lymphatic Vessels. (October 2014)

• Record Type: Journal Article
• Authors: Nizamutdinova, Irina Tsoy; Maejima, Daisuke; Nagai, Takashi; Bridenbaugh, Eric; Thangaswamy, Sangeetha; Chatterjee, Victor; Meininger, Cynthia J.; Gashev, Anatoliy A.
• Is Part Of: Microcirculation. Volume 21:Number 7(2014:Oct.)
• Page Start: 640
• Page End: 648
• Publication Date: 2014-10
• Subjects: Biological transport -- Periodicals; Microcirculation -- Physiology -- Periodicals; 612.135
• DOI: 10.1111/micc.12143
• Languages: English
• ISSNs: 1073-9688

Abstract

<abstract abstract-type="main" id="micc12143-abs-0001"> <title>Abstract</title> <sec id="micc12143-sec-0001" sec-type="section"> <title>Objectives</title> <p>The knowledge of the basic principles of lymphatic function, still remains, to a large degree, rudimentary and will require significant research efforts. Recent studies of the physiology of the MLVs suggested the presence of an EDRF other than NO. In this study, we tested the hypothesis that lymphatic endothelium‐derived histamine relaxes MLVs.</p> </sec> <sec id="micc12143-sec-0002" sec-type="section"> <title>Methods</title> <p>We measured and analyzed parameters of lymphatic contractility in isolated and pressurized rat MLVs under control conditions and after pharmacological blockade of NO by <sc>l</sc>‐NAME (100 <italic>μ</italic>M) or/and histamine production by <italic>α</italic>‐MHD (10 <italic>μ</italic>M). Effectiveness of <italic>α</italic>‐MHD was confirmed immunohistochemically. We also used immunohistochemical labeling and Western blot analysis of the histamine‐producing enzyme, HDC. In addition, we blocked HDC protein expression in MLVs by transient transfection with vivo‐morpholino oligos.</p> </sec> <sec id="micc12143-sec-0003" sec-type="section"> <title>Results</title> <p>We found that only combined pharmacological blockade of NO and histamine production completely eliminates flow‐dependent relaxation of lymphatic vessels, thus confirming a role for histamine as an EDRF in MLVs. We also confirmed the presence of HDC and histamine inside lymphatic endothelial cells.</p> </sec> <sec id="micc12143-sec-0004" sec-type="section"> <title>Conclusions</title> <p>This study supports a role for histamine as an EDRF in MLVs.</p> </sec> </abstract>