Exploring the immediate and long‐term impact on bacterial communities in soil amended with animal and urban organic waste fertilizers using pyrosequencing and screening for horizontal transfer of antibiotic resistance. Issue 1 (28th August 2014)

• Record Type: Journal Article
• Authors: Riber, Leise; Poulsen, Pernille H.B.; Al‐Soud, Waleed A.; Skov Hansen, Lea B.; Bergmark, Lasse; Brejnrod, Asker; Norman, Anders; Hansen, Lars H.; Magid, Jakob; Sørensen, Søren J.
• Is Part Of: FEMS microbiology ecology. Volume 90:Issue 1(2014)
• Page Start: 206
• Page End: 224
• Publication Date: 2014-08-28
• Subjects: Microbial ecology -- Periodicals; Microbiology -- Periodicals; 579.17
• DOI: 10.1111/1574-6941.12403
• Languages: English
• ISSNs: 0168-6496

Abstract

<abstract abstract-type="main" id="fem12403-abs-0001"> <title>Abstract</title> <p>We investigated immediate and long‐term effects on bacterial populations of soil amended with cattle manure, sewage sludge or municipal solid waste compost in an ongoing agricultural field trial. Soils were sampled in weeks 0, 3, 9 and 29 after fertilizer application. <italic>Pseudomonas</italic> isolates were enumerated, and the impact on soil bacterial community structure was investigated using 16S rRNA amplicon pyrosequencing. Bacterial community structure at phylum level remained mostly unaffected. <italic>Actinobacteria</italic>, <italic> Proteobacteria</italic> and <italic>Chloroflexi</italic> were the most prevalent phyla significantly responding to sampling time. Seasonal changes seemed to prevail with decreasing bacterial richness in week 9 followed by a significant increase in week 29 (springtime). The <italic>Pseudomonas</italic> population richness seemed temporarily affected by fertilizer treatments, especially in sludge‐ and compost‐amended soils. To explain these changes, prevalence of antibiotic‐ and mercury‐resistant pseudomonads was investigated. Fertilizer amendment had a transient impact on the resistance profile of the soil community; abundance of resistant isolates decreased with time after fertilizer application, but persistent strains appeared multiresistant, also in unfertilized soil. Finally, the ability of a <italic>P. putida</italic> strain to take up resistance genes from indigenous soil bacteria by horizontal gene transfer was present only in week 0, indicating a temporary increase in prevalence of transferable antibiotic resistance genes.</p> </abstract>