MOLECULAR CLONING AND CHARACTERIZATION OF A SID‐1‐LIKE GENE IN Plutella xylostella. Issue 3 (18th August 2014)

• Record Type: Journal Article
• Authors: Wang, Huidong; Gong, Liang; Qi, Jiangwei; Hu, Meiying; Zhong, Guohua; Gong, Liang
• Is Part Of: Archives of insect biochemistry and physiology. Volume 87:Issue 3(2014:Nov.)
• Page Start: 164
• Page End: 176
• Publication Date: 2014-08-18
• Subjects: Insects -- Physiology -- Periodicals; Insect biochemistry -- Periodicals; 595.701572
• DOI: 10.1002/arch.21189
• Languages: English
• ISSNs: 0739-4462

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>RNA interference (RNAi) signal can spread from the point where the double‐stranded RNA (dsRNA) was initially applied to other cells or tissues. SID‐related genes in Caenorhabditis elegans help in the spreading of this signal. However, the mechanisms of systemic RNAi are still not unveiled in insects. In this study, we cloned a full‐length cDNA of sid‐1‐like gene, Pxylsid‐1, from Plutella xylostella that contains 1, 047 bp opening reading frame encoding a putative protein of 348 amino acids. This transcript is very much similar to the sil‐1 in Bombyx mori (68.8%). The higher expression levels of Pxylsid‐1 were found at the adult and fourth‐instar stages compared to the second‐instar stage with 21.48‐ and 10.36‐fold increase, respectively. Its expression levels in different tissues were confirmed with the highest expression in the hemolymph, which showed 21.09‐fold increase than the midgut; however it was lower in other tissues. The result of RNAi by feeding bacterially expressed dsRNA targeting <italic>Pxylace‐1</italic>, which showed that the mRNA level of <italic>Pxylace‐1</italic> decreased by 34.52 and 64.04% after 36‐ and 72‐h treatment, respectively. However, the mRNA level of Pxylsid‐1 was not significantly induced when the <italic>Pxylace‐1</italic> was downregulated. Furthermore, we found that downregulation of Pxylsid‐1 did not affect the RNAi effect of <italic>Pxylace‐1</italic>. Hence, the Pxylsid‐1 may not be involved in absorption of dsRNA from the midgut fluid. A further study is needed to uncover the function of Pxylsid‐1.</p> </abstract>