Stable mixed hematopoietic chimerism permits tolerance of vascularized composite allografts across a full major histocompatibility mismatch in swine. (28th August 2014)

• Record Type: Journal Article
• Authors: Mathes, David W.; Solari, Mario G.; Gazelle, Guy Scott; Butler, Peter E. M.; Wu, Anette; Nazzal, Adam; Nielsen, Gunnlauger P.; Huang, Christene A.; Sachs, David H.; Lee, Wei Ping Andrew; Randolph, Mark A.
• Is Part Of: Transplant international. Volume 27:Number 10(2014:Oct.)
• Page Start: 1086
• Page End: 1096
• Publication Date: 2014-08-28
• Subjects: Transplantation of organs, tissues, etc -- Periodicals; 617.95405
• DOI: 10.1111/tri.12380
• Languages: English
• ISSNs: 0934-0874

Abstract

<abstract abstract-type="main" id="tri12380-abs-0001"> <title>Summary</title> <p>This study tested the hypothesis that vascularized composite allografts (VCA) could be accepted in a robust model of hematopoietic chimerism by injecting allogeneic bone marrow cells (BMC) into swine fetuses. Outbred Yorkshire sows and boars were screened to ensure the absence of the major histocompatibility (MHC) allele SLA<sup>cc</sup> of inbred MGH miniature swine and then mated. Bone marrow harvested from an SLA<sup>cc</sup> swine donor was T‐cell depleted and injected intravenously into the fetuses between days 50–55 of gestation. After birth, the piglets were studied with flow cytometry to detect donor cells and mixed lymphocyte reactions (MLR) and cell‐mediated lympholysis (CML) assays to assess their response to donor. Donor‐matched VCAs from SLA<sup>cc</sup> donors were performed on four chimeric and two nonchimeric swine. The results showed donor cell engraftment and multilineage macrochimerism after the <italic>in utero</italic> transplantation of adult BMC, and chimeric animals were unresponsive to donor antigens <italic>in vitro</italic>. Both control VCAs were rejected by 21 days and were alloreactive. Chimeric animals accepted the VCAs and never developed antidonor antibodies or alloreactivity to donor. These results confirm that the intravascular, <italic>in utero</italic> transplantation of adult BMC leads to donor cell chimerism and donor‐specific tolerance of VCAs across a <italic>full MHC barrier</italic> in this animal model.</p> </abstract>