Vascular endothelial growth factor A polymorphism and risk of Kaposi's sarcoma herpesvirus viremia in kidney allograft recipients. Issue 5 (14th August 2014)

• Record Type: Journal Article
• Authors: Alkharsah, K.R.; Alzahrani, A.J.; Obeid, O.E.; El‐Harith, E.‐H.A.; Guella, A.; Mohamed, E.A.; Haykal, A.H.; Stuhrmann, M.; Al‐Ali, A.K.
• Is Part Of: Transplant infectious disease. Volume 16:Issue 5(2014)
• Page Start: 783
• Page End: 789
• Publication Date: 2014-08-14
• Subjects: Transplantation of organs, tissues, etc -- Complications -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 617.01
• DOI: 10.1111/tid.12277
• Languages: English
• ISSNs: 1398-2273

Abstract

<abstract abstract-type="main" id="tid12277-abs-0001"> <title>Abstract</title> <sec id="tid12277-sec-0001" sec-type="section"> <title>Background</title> <p>Kaposi's sarcoma herpesvirus (KSHV) causes Kaposi's sarcoma (KS), primary effusion lymphoma, and multicentric Castleman's disease in immunocompromised patients including allograft recipients. Detection of KSHV DNA in blood, as well as host genetic polymorphisms has been found to be associated with an increased risk for KS. We investigated an association between single nucleotide polymorphisms (SNPs) in vascular endothelial growth factor A (<italic>VEGFA</italic>) gene region and KSHV viremia in kidney transplant recipients (KTR) in Saudi Arabia.</p> </sec> <sec id="tid12277-sec-0002" sec-type="section"> <title>Methods</title> <p>In total, 152 KTR who have survived kidney transplantation for at least 6 months were included in the study. KSHV viremia was determined by real‐time polymerase chain reaction (PCR). Genotyping of SNPs in the <italic>VEGFA</italic> region was performed by PCR and direct sequencing, as well as by restriction fragment length polymorphism.</p> </sec> <sec id="tid12277-sec-0003" sec-type="section"> <title>Results</title> <p>KSHV DNA was detected in 28.9% (<italic>n</italic> = 44) of the study population. The A‐allele at position C172A <italic>VEGFA</italic> gene promoter region was found to be associated with KSHV viremia (odd ratio [OR] = 4.8, <italic>P</italic> = 0.005). In addition, the G‐allele at position C+405G in the 5′‐untranslated region was associated with KSHV viremia in women, but not in men (OR = 3.98, <italic>P</italic> = 0.004).</p> </sec> <sec id="tid12277-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Our results suggest an association of <italic>VEGFA</italic> polymorphisms with KSHV viremia among KTR in this study population. A limitation of our study is that the results can only be predicated for patients 6 months after kidney transplantation and should be validated in another cohort with larger sample size.</p> </sec> </abstract>