Compositional changes of human hair melanin resulting from bleach treatment investigated by nanoscale secondary ion mass spectrometry. Issue 4 (8th February 2014)

• Record Type: Journal Article
• Authors: Kojima, Toru; Yamada, Hiromi; Isobe, Mitsuru; Yamamoto, Toshihiko; Takeuchi, Miyuki; Aoki, Dan; Matsushita, Yasuyuki; Fukushima, Kazuhiko
• Is Part Of: Skin research and technology. Volume 20:Issue 4(2014)
• Page Start: 416
• Page End: 421
• Publication Date: 2014-02-08
• Subjects: Skin -- Research -- Periodicals; Skin -- Diseases -- Periodicals; Skin -- Physiology -- Periodicals; 616.5
• DOI: 10.1111/srt.12133
• Languages: English
• ISSNs: 0909-752X

Abstract

<abstract abstract-type="main" id="srt12133-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="srt12133-sec-0001" sec-type="section"> <title>Background/purpose</title> <p>It is important to understand the influence of bleach treatment on human hair because it is one of the most important chemical treatments in hair cosmetic processes. A comparison of the elemental composition of melanin between virgin hair and bleached hair would provide important information about the structural changes of melanin. To investigate the elemental composition of melanin granules in virgin black hair and bleached hair, these hair cross‐sections are analyzed by using a nanoscale secondary ion mass spectrometry (NanoSIMS).</p> </sec> <sec id="srt12133-sec-0002" sec-type="section"> <title>Methods</title> <p>The virgin black hair and bleached hair samples were embedded in resin and smooth hair cross‐sections were obtained using an ultramicrotome. NanoSIMS measurements were performed using a Cs<sup>+</sup> primary ion beam to detect negative secondary ions.</p> </sec> <sec id="srt12133-sec-0003" sec-type="section"> <title>Results</title> <p>More intensive <sup>16</sup>O<sup>−</sup> ions were detected from the melanin granules of bleached hair than from those of virgin black hair in NanoSIMS <sup>16</sup>O<sup>−</sup> ion image. In addition, it was indicated that <sup>16</sup>O<sup>−</sup> ion intensity and <sup>16</sup>O<sup>‐</sup>/<sup>12</sup>C<sup>14</sup>N<sup>−</sup> ion intensity ratio of melanin granules in bleached hair were higher than those in virgin black hair.</p> </sec> <sec id="srt12133-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Nanoscale secondary ion mass spectrometry analysis of the cross‐sections of virgin black hair and bleached hair indicated that the oxygen content in melanin granules was increased by bleach treatment.</p> </sec> </abstract>