Identification of AFLP and SSR markers linked with the male fertility restorer gene of CMS 06J45 in heading Chinese cabbage (Brassica rapa L. ssp. pekinensis). Issue 5 (17th June 2014)

• Record Type: Journal Article
• Authors: Xu, Xiaoyong; Sun, Xilu; Zhang, Yu; Zhang, Lugang; Fang, Zhiyuan; Friedt, W.
• Is Part Of: Plant breeding. Volume 133:Issue 5(2014)
• Page Start: 615
• Page End: 619
• Publication Date: 2014-06-17
• Subjects: Plant breeding -- Periodicals; 631.52
• DOI: 10.1111/pbr.12200
• Languages: English
• ISSNs: 0179-9541

Abstract

<abstract abstract-type="main" id="pbr12200-abs-0001"> <title>Abstract</title> <p>In this study, AFLP and SSR techniques were combined with the bulk segregant analysis (BSA) method to map the restorer gene <italic>BrRfp</italic> using an F₂‐segregating population comprising 258 individuals developed by crossing the <italic>polima</italic> (<italic>pol</italic>)‐like cytoplasmic male sterility (CMS) line 06J45 and the restorer line 01S325 of heading Chinese cabbage. A survey of 2048 AFLP primer pairs identified 21 polymorphic fragments, approximately half of which exhibited high similarity with the A09 chromosome sequence of <italic>Brassica rapa</italic> in the <italic>Brassica</italic> database (BRAD). Based on the genome sequence, three specific AFLP fragments linked with <italic>BrRfp</italic> were successfully converted into sequence‐characterized amplified region (SCAR) markers, named SC1233, SC2673 and SC2141. Subsequently, 178 pairs of SSR primers were redesigned for further screening, with five producing polymorphic amplification patterns. Linkage analysis showed that these markers were distributed along both sides of the <italic>BrRfp</italic> gene, with two markers, SSR03 and SSR2528, co‐segregating with the <italic>BrRfp</italic> locus in the F₂ population. These results may be valuable for marker‐assisted selection and map‐based cloning in heading Chinese cabbage.</p> </abstract>