N‐Terminal Pro‐C‐Natriuretic Peptide and Cytokine Kinetics in Dogs with Endotoxemia. (23rd July 2014)

• Record Type: Journal Article
• Authors: Floras, A.N.K.; Holowaychuk, M.K.; Bienzle, D.; Bersenas, A.M.E.; Sharif, S.; Harvey, T.; Nordone, S.K.; Wood, G.A.
• Is Part Of: Journal of veterinary internal medicine. Volume 28:Number 5(2014:Sep./Oct.)
• Page Start: 1447
• Page End: 1453
• Publication Date: 2014-07-23
• Subjects: Veterinary medicine -- Periodicals; 636.0896
• DOI: 10.1111/jvim.12409
• Languages: English
• ISSNs: 0891-6640

Abstract

<abstract abstract-type="main" id="jvim12409-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jvim12409-sec-0001" sec-type="section"> <title>Background</title> <p>Serum N‐terminal pro‐C‐natriuretic peptide (NT‐proCNP) concentration at hospital admission has sufficient sensitivity and specificity to differentiate naturally occurring sepsis from nonseptic systemic inflammatory response syndrome (SIRS). However, little is known about serum NT‐proCNP concentrations in dogs during the course of sepsis.</p> </sec> <sec id="jvim12409-sec-0002" sec-type="section"> <title>Objective</title> <p>To determine serum NT‐proCNP and cytokine kinetics in dogs with endotoxemia, a model of canine sepsis.</p> </sec> <sec id="jvim12409-sec-0003" sec-type="section"> <title>Samples</title> <p>Eighty canine serum samples.</p> </sec> <sec id="jvim12409-sec-0004" sec-type="section"> <title>Methods</title> <p>Eight healthy adult Beagles were randomized to receive <italic>Escherichia coli</italic> lipopolysaccharide (LPS, 5 μg/kg) or placebo (0.9% NaCl) as a single IV dose in a randomized crossover study. Serum collected at 0, 1, 2, 4, and 24 hours was stored at −80°C for batch analysis. Serum NT‐proCNP was measured by ELISA and 13 cytokines and chemokines by multiplex magnetic bead‐based assay.</p> </sec> <sec id="jvim12409-sec-0005" sec-type="section"> <title>Results</title> <p>Serum NT‐proCNP concentrations did not differ significantly between LPS‐ and placebo‐treated dogs at any time. When comparing serum cytokine concentrations, LPS‐treated dogs had higher interleukin‐6 (IL‐6), IL‐10, TNF‐α and KC‐like at 1, 2, and 4 hours; higher CCL2 at 1, 2, 4, and 24 hours; and higher IL‐8 and CXCL10 at 4 hours compared to placebo‐treated dogs. There were no differences in serum GM‐CSF, IFN‐γ, IL‐2, IL‐7, IL‐15 or IL‐18 between LPS‐ and placebo‐treated dogs.</p> </sec> <sec id="jvim12409-sec-0006" sec-type="section"> <title>Conclusions and Clinical Importance</title> <p>Serum NT‐proCNP concentration does not change significantly in response to LPS administration in healthy dogs. Certain serum cytokine and chemokine concentrations are significantly increased within 1–4 hours after LPS administration and warrant further investigation as tools for the detection and management of sepsis in dogs.</p> </sec> </abstract>