Expression of placenta growth factor, soluble fms‐like tyrosine kinase‐1, metal‐responsive transcription factor‐1, heme oxygenase 1 and hypoxia inducible factor‐1α mRNAs in pre‐eclampsia placenta and the effect of pre‐eclampsia sera on their expression of choriocarcinoma cells. Issue 10 (11th August 2014)

• Record Type: Journal Article
• Authors: Maebayashi (Asanuma), Aki; Yamamoto, Tatsuo; Azuma, Hiromitu; Kato, Erina; Yamamoto, Noriko; Murase, Takayuki; Chishima, Fumihisa; Suzuki, Manami
• Is Part Of: Journal of obstetrics and gynaecology research. Volume 40:Issue 10(2014:Oct.)
• Page Start: 2095
• Page End: 2103
• Publication Date: 2014-08-11
• Subjects: Gynecology -- Periodicals; Obstetrics -- Periodicals; 618.1005
• DOI: 10.1111/jog.12462
• Languages: English
• ISSNs: 1341-8076

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="jog12462-sec-0001" sec-type="section"> <title>Aim</title> <p>We studied the effect of pre‐eclampsia sera on the expression of placenta growth factor (PlGF), soluble fms‐like tyrosine kinase‐1 (sFlt‐1), metal‐responsive transcription factor‐1 (MTF‐1), heme oxygenase 1 (HO‐1) and hypoxia inducible factor‐1α (HIF‐1α) mRNAs in JEG‐3 cells (trophoblast‐derived cells) and placenta from pre‐eclampsia patients to investigate pre‐eclampsia pathophysiology.</p> </sec> <sec id="jog12462-sec-0002" sec-type="section"> <title>Material and Methods</title> <p>Placenta and serum samples were taken from pre‐eclampsia and normal pregnancy patients. JEG‐3 cells were cultured with pre‐eclampsia and normal pregnant sera in 24‐well tissue culture plates. RNA was purified from placental trophoblast cells and JEG‐3 cells 24 h after incubation. The expression of mRNA was measured using real‐time polymerase chain reaction.</p> </sec> <sec id="jog12462-sec-0003" sec-type="section"> <title>Results</title> <p>The expression of sFlt‐1 mRNA increased, and that of PlGF and HO‐1 mRNA decreased in JEG‐3 cells after incubation with pre‐eclampsia sera. The expression of PlGF mRNA decreased, and that of sFlt‐1mRNA increased in pre‐eclampsia placenta. The expression of MTF‐1 and HO‐1 mRNA decreased. A correlation was found between PlGF mRNA expression and the expression of MTF‐1 and HIF‐1α mRNA. A correlation between sFlt‐1 and HIF‐1α mRNA expression was also found.</p> </sec> <sec id="jog12462-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Changes in PlGF mRNA expression in pre‐eclampsia placenta may relate to serum factors and the expression of MTF‐1 and HIF‐α mRNA. Changes in sFlt‐1mRNA expression may relate to serum factors and the expression of HIF‐α mRNA. We suggest that serum factors play a role in PlGF and sFlt‐1 expression in pre‐eclampsia placenta.</p> </sec> </abstract>