DNA vaccination by intradermal electroporation induces long‐lasting immune responses in rhesus macaques. Issue 5 (9th May 2014)

• Record Type: Journal Article
• Authors: Kulkarni, Viraj; Rosati, Margherita; Jalah, Rashmi; Ganneru, Brunda; Alicea, Candido; Yu, Lei; Guan, Yongjun; LaBranche, Celia; Montefiori, David C.; King, Alan D.; Valentin, Antonio; Pavlakis, George N.; Felber, Barbara K.
• Is Part Of: Journal of medical primatology. Volume 43:Issue 5(2014)
• Page Start: 329
• Page End: 340
• Publication Date: 2014-05-09
• Subjects: Primates -- Periodicals; Primates -- Diseases -- Periodicals; Medicine, Experimental -- Periodicals; 616.02738
• DOI: 10.1111/jmp.12123
• Languages: English
• ISSNs: 0047-2565

Abstract

<abstract abstract-type="main" id="jmp12123-abs-0001"> <title>Abstract</title> <sec id="jmp12123-sec-0001" sec-type="section"> <title>Background</title> <p>A desirable HIV vaccine should induce protective long‐lasting humoral and cellular immune responses.</p> </sec> <sec id="jmp12123-sec-0002" sec-type="section"> <title>Methods</title> <p>Macaques were immunized by <italic>env </italic>DNA, selected from a panel of recently transmitted SIVmac251 Env using intradermal electroporation as vaccine delivery method and magnitude, breadth and longevity of humoral and cellular immune responses.</p> </sec> <sec id="jmp12123-sec-0003" sec-type="section"> <title>Results</title> <p>The macaques developed high, long‐lasting humoral immune responses with neutralizing capacity against homologous and heterologous Env. The avidity of the antibody responses was also preserved over 1‐year follow‐up. Analysis of cellular immune responses demonstrated induction of Env‐specific memory T cells harboring granzyme B, albeit their overall levels were low. Similar to the humoral responses, the cellular immunity was persistent over the ~1‐year follow‐up.</p> </sec> <sec id="jmp12123-sec-0004" sec-type="section"> <title>Conclusion</title> <p>These data show that vaccination by this intradermal DNA delivery regimen is able to induce potent and durable immune responses in macaques.</p> </sec> </abstract>