Proteomic and genomic analyses suggest the association of apolipoprotein C1 with abdominal aortic aneurysm. Issue 10 (19th May 2014)

• Record Type: Journal Article
• Authors: Moxon, Joseph V.; Liu, Dawei; Moran, Corey S.; Crossman, David J.; Krishna, Smriti M.; Yonglitthipagon, Ponlapat; Emeto, Theophilus I.; Morris, Dylan R.; Padula, Matthew P.; Mulvenna, Jason P.; Rush, Catherine M.; Golledge, Jonathan; Bendixen, E.
• Is Part Of: Proteomics. Volume 8:Issue 10(2014)
• Page Start: 762
• Page End: 772
• Publication Date: 2014-05-19
• Subjects: Proteomics -- Periodicals; 572.605
• DOI: 10.1002/prca.201300119
• Languages: English
• ISSNs: 1862-8346

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="prca1536-sec-0010" sec-type="section"> <title>Purpose</title> <p>Abdominal aortic aneurysm (AAA) is an important cause of mortality in the elderly. Mouse models are widely used to investigate AAA pathogenesis but their suitability for biomarker discovery is unexplored.</p> </sec> <sec id="prca1536-sec-0020" sec-type="section"> <title>Experimental design</title> <p>We conducted a three‐phase study. Phase 1: Aortas from angiotensin‐II‐infused apolipoprotein E deficient (ApoE<sup>−/−</sup>) mice with and without AAA were assessed via iTRAQ and analyzed in silico to identify potential circulating markers. Microarray data from ApoE<sup>−/−</sup> mice and human patients were analyzed in parallel. Phase 2: Putative markers were compared between datasets to shortlist common candidates. Phase 3: The relationship of two shortlisted markers and AAA presence was assessed.</p> </sec> <sec id="prca1536-sec-0030" sec-type="section"> <title>Results</title> <p>iTRAQ identified eight proteins with biomarker potential. Microarray data identified 72 and 96 potential biomarkers from ApoE<sup>−/−</sup> mice and human patients, respectively. All three datasets suggested apolipoprotein C1 (ApoC1) as a marker for AAA; microarray data identified matrix metalloproteinase 9 (MMP9) as a second potential marker. Plasma ApoC1 and MMP9 concentrations positively correlated with AAA diameter in ApoE<sup>−/−</sup> mice.</p> </sec> <sec id="prca1536-sec-0040" sec-type="section"> <title>Conclusions and clinical relevance</title> <p>ApoC1 may be a novel biomarker for AAA.</p> </sec> </abstract>