Serum tri‐ and tetra‐antennary N‐glycan is a potential predictive biomarker for castration‐resistant prostate cancer. Issue 15 (23rd August 2014)

• Record Type: Journal Article
• Authors: Ishibashi, Yusuke; Tobisawa, Yuki; Hatakeyama, Shingo; Ohashi, Tetsu; Tanaka, Masakazu; Narita, Shintaro; Koie, Takuya; Habuchi, Tomonori; Nishimura, Shin‐Ichiro; Ohyama, Chikara; Yoneyama, Tohru
• Is Part Of: Prostate. Volume 74:Issue 15(2014)
• Page Start: 1521
• Page End: 1529
• Publication Date: 2014-08-23
• Subjects: Prostate -- Diseases -- Periodicals; 616
• DOI: 10.1002/pros.22869
• Languages: English
• ISSNs: 0270-4137

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="pros22869-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p>The U.S. FDA has approved several novel systemic agents including abiraterone acetate and taxoid cabazitaxel for metastatic castration‐resistant prostate cancer (CRPC) result in a complicated decision‐making while selecting an appropriate treatment. Therefore, a predictive biomarker for CRPC would provide useful information to physicians. The aim of this study is to evaluate the diagnostic potential of serum <italic>N</italic>‐glycan profiling in CRPC.</p> </sec> <sec id="pros22869-sec-0002" sec-type="section"> <title>METHODS</title> <p>Serum <italic>N</italic>‐glycomics was performed in 80 healthy volunteers and 286 benign prostatic hyperplasia, 258 early‐stage PC, 46 PC with androgen deprivation therapy (ADT), and 68 CRPC patients using the glycoblotting method. A total of 36 types of <italic>N</italic>‐glycan levels in each patient were analyzed using logistic regression analysis and receiver operating characteristic curves. We also examined the expression of <italic>N</italic>‐glycan branching enzyme genes in PC cell lines using quantitative RT‐PCR.</p> </sec> <sec id="pros22869-sec-0003" sec-type="section"> <title>RESULTS</title> <p>We observed that tri‐ and tetra‐antennary <italic>N</italic>‐glycans were significantly higher in CRPC patients than in any other groups. The longitudinal follow‐up of tri‐ and tetra‐ antennary <italic>N</italic>‐glycan levels revealed that one PC with ADT patient showed an increase that was more than the cut‐off level and two consecutive increases in tri‐ and tetra‐antennary <italic>N</italic>‐glycan levels 3 months apart; resulted in biochemical recurrence despite the castrate level of testosterone, and the patient was defined as CRPC. Expression of <italic>N</italic>‐glycan branching enzyme genes were significantly upregulated in CRPC cell lines.</p> </sec> <sec id="pros22869-sec-0004" sec-type="section"> <title>CONCLUSIONS</title> <p>These results suggest that the overexpression of tri‐ and tetra‐antennary <italic>N</italic>‐glycan may be associated with the castration‐resistant status in PC and may be a potential predictive biomarker for CRPC. <italic>Prostate 74:1521–1529, 2014</italic>. © 2014 Wiley Periodicals, Inc.</p> </sec> </abstract>