Orthotopic Wilms tumor xenografts derived from cell lines reflect limited aspects of tumor morphology and clinical characteristics. Issue 11 (7th July 2014)

• Record Type: Journal Article
• Authors: Mengelbier, Linda Holmquist; Bexell, Daniel; Sehic, Daniel; Ciornei, Cristina D.; Gisselsson, David
• Is Part Of: Pediatric blood & cancer. Volume 61:Issue 11(2014:Nov.)
• Page Start: 1949
• Page End: 1954
• Publication Date: 2014-07-07
• Subjects: Tumors in children -- Periodicals; Blood -- Diseases -- Periodicals; Cancer in children -- Periodicals; 618.92
• DOI: 10.1002/pbc.25131
• Languages: English
• ISSNs: 1545-5009

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="pbc25131-sec-0001" sec-type="section"> <title>Background</title> <p>Wilms tumor (WT) is a pediatric tumor of the kidney, the treatment of which includes heavy chemotherapy. Affected children would likely benefit from more targeted therapies with limited side effects. Establishment of relevant orthotopic WT xenografts is important to better understand mechanisms of WT growth and for preclinical drug testing.</p> </sec> <sec id="pbc25131-sec-0002" sec-type="section"> <title>Procedure</title> <p>Here we established and characterized orthotopic xenografts from WT cell lines WiT49, CCG‐99‐11, and WT‐CLS1 to ascertain in what aspects each of them recapitulated WT histology, immunophenotype, invasion, and metastatic spread.</p> </sec> <sec id="pbc25131-sec-0003" sec-type="section"> <title>Results</title> <p>WiT49 xenografts recapitulated near triphasic WTs with clear WT1 staining and anaplastic features, but with tumor restricted to the kidney. On the contrary both CCG‐99‐11 and WT‐CLS1 xenografts conveyed metastatic disease. CCG‐99‐11 showed a blastemal phenotype whereas WT‐CLS1 xenografts did not properly reflect any specific WT subtype.</p> </sec> <sec id="pbc25131-sec-0004" sec-type="section"> <title>Conclusions</title> <p>From the three tested cell lines, orthotopic WiT49 xenografts best reflect the triphasic pattern of classical WT. Pediatr Blood Cancer 2014;61:1949–1954. © 2014 Wiley Periodicals, Inc.</p> </sec> </abstract>