Systemic NK cell ablation attenuates intra‐abdominal adipose tissue macrophage infiltration in murine obesity. (24th June 2014)

• Record Type: Journal Article
• Authors: O'Rourke, Robert W.; Meyer, Kevin A.; Neeley, Christopher K.; Gaston, Garen D.; Sekhri, Palak; Szumowski, Marek; Zamarron, Brian; Lumeng, Carey N.; Marks, Daniel L.
• Is Part Of: Obesity. Volume 22:Number 10(2014:Oct.)
• Page Start: 2109
• Page End: 2114
• Publication Date: 2014-06-24
• Subjects: Obesity -- Periodicals; 616.398005
• DOI: 10.1002/oby.20823
• Languages: English
• ISSNs: 1930-7381

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="oby20823-sec-0001" sec-type="section"> <title>Objective</title> <p>Natural killer (NK) cells are understudied in the context of metabolic disease and obesity. The goal of this study was to define the effect of NK cell ablation on systemic inflammation and glucose homeostasis in murine obesity.</p> </sec> <sec id="oby20823-sec-0002" sec-type="section"> <title>Methods</title> <p>A transgenic murine model was used to study the effect of NK cell ablation on systemic inflammation and glucose homeostasis in the context of diet‐induced obesity using flow cytometry, QRTPCR, and glucose tolerance and insulin sensitivity testing.</p> </sec> <sec id="oby20823-sec-0003" sec-type="section"> <title>Results</title> <p>NK cell ablation achieved a three to fourfold decrease in NK cells but had no effect on T‐cell levels in adipose tissues and spleen. NK cell ablation was associated with decreased total macrophage infiltration in intra‐abdominal adipose tissue, but macrophage infiltration in subcutaneous adipose tissue and spleen was unaffected. NK cell ablation was associated with modest improvement in insulin sensitivity but had no effect on tissue transcript levels of inflammatory cytokines.</p> </sec> <sec id="oby20823-sec-0004" sec-type="section"> <title>Conclusions</title> <p>NK cells play a role in promoting intra‐abdominal adipose tissue macrophage infiltration and systemic insulin resistance in obesity.</p> </sec> </abstract>