Upregulation of intestinal glucose transporters after Roux‐en‐Y gastric bypass to prevent carbohydrate malabsorption. (2nd July 2014)

• Record Type: Journal Article
• Authors: Nguyen, Nam Q.; Debreceni, Tamara L.; Bambrick, Jenna E.; Chia, Bridgette; Deane, Adam M.; Wittert, Gary; Rayner, Chris K.; Horowitz, Michael; Young, Richard L.
• Is Part Of: Obesity. Volume 22:Number 10(2014:Oct.)
• Page Start: 2164
• Page End: 2171
• Publication Date: 2014-07-02
• Subjects: Obesity -- Periodicals; 616.398005
• DOI: 10.1002/oby.20829
• Languages: English
• ISSNs: 1930-7381

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="oby20829-sec-0001" sec-type="section"> <title>Objective</title> <p>To determine the effect of Roux‐en‐Y gastric bypass (RYGB) on the expression of intestinal sweet taste receptors (STRs), glucose transporters (GTs), glucose absorption, and glycemia.</p> </sec> <sec id="oby20829-sec-0002" sec-type="section"> <title>Methods</title> <p>Intestinal biopsies were collected for mRNA expression of STR (T1R2) and GTs (SGLT‐1 and GLUT2) from 11 non‐diabetic RYGB, 13 non‐diabetic obese, and 11 healthy subjects, at baseline and following a 30 min small intestinal (SI) glucose infusion (30 g/150 ml water with 3 g 3‐<italic>O</italic>‐methyl‐<sc>d</sc>‐glucopyranose (3‐OMG)). Blood glucose, plasma 3‐OMG, and insulin were measured for 270 min.</p> </sec> <sec id="oby20829-sec-0003" sec-type="section"> <title>Results</title> <p>In RYGB patients, expression of both GTs was ∼2‐fold higher at baseline and after glucose infusion than those of morbidly obese or healthy subjects (<italic>P</italic> < 0.001). STR expressions were comparable amongst the groups. Peak plasma 3‐OMG in both RYGB (<italic>r</italic> = 0.69, <italic>P</italic> = 0.01) and obese (<italic>r</italic> = 0.72, <italic>P</italic> = 0.005) correlated with baseline expression of SGLT‐1, as was the case with peak blood glucose in RYGB subjects (<italic>r</italic> = 0.69, <italic>P</italic> = 0.02).</p> </sec> <sec id="oby20829-sec-0004" sec-type="section"> <title>Conclusions</title> <p>The upregulated intestinal GTs in RYGB patients are associated with increased glucose absorption when glucose is delivered at a physiological rate, suggesting a molecular adaptation to prevent carbohydrate malabsorption from rapid intestinal transit after RYGB.</p> </sec> </abstract>