Toward hyperpolarized molecular imaging of HIV: synthesis and longitudinal relaxation properties of 15N‐Azidothymidine. (25th August 2014)

• Record Type: Journal Article
• Authors: Shchepin, Roman V.; Chekmenev, Eduard Y.
• Is Part Of: Journal of labelled compounds & radiopharmaceuticals. Volume 57:Number 10(2014)
• Page Start: 621
• Page End: 624
• Publication Date: 2014-08-25
• Subjects: Tracers (Chemistry) -- Periodicals; Radiopharmaceuticals -- Periodicals; 615.8424
• DOI: 10.1002/jlcr.3220
• Languages: English
• ISSNs: 0362-4803

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Previously unreported ¹⁵N labeled Azidothymidine (AZT) was prepared as an equimolar mixture of two isotopomers: 1‐¹⁵N‐AZT and 3‐¹⁵N‐AZT. Polarization decay of ¹⁵N NMR signal was studied in high (9.4 T) and low (~50 mT) magnetic fields. ¹⁵N <italic>T</italic>₁ values were 45 ± 5 s (1‐¹⁵N‐AZT) and 37 ± 2 s (3‐¹⁵N‐AZT) at 9.4 T, and 140 ± 16 s (3‐¹⁵N‐AZT) at 50 mT. ¹⁵N‐AZT can be potentially ¹⁵N hyperpolarized by several methods. These sufficiently long ¹⁵N‐AZT <italic>T</italic>₁ values potentially enable hyperpolarized <italic>in vivo</italic> imaging of ¹⁵N‐AZT, because of the known favorable efficient (i.e., of the time scale shorter than the longest reported here ¹⁵N <italic>T</italic>₁) kinetics of uptake of injected AZT. Therefore, 3‐¹⁵N‐AZT can be potentially used for HIV molecular imaging using hyperpolarized magnetic resonance imaging.</p> </abstract>