Evaluation of analytical performance and reliability of direct nanoLC‐nanoESI‐high resolution mass spectrometry for profiling the (xeno)metabolome. (29th September 2014)

• Record Type: Journal Article
• Authors: Chetwynd, Andrew J.; David, Arthur; Hill, Elizabeth M.; Abdul‐Sada, Alaa
• Is Part Of: Journal of mass spectrometry. Volume 49:Number 10(2014:Oct.)
• Page Start: 1063
• Page End: 1069
• Publication Date: 2014-09-29
• Subjects: Mass spectrometry -- Periodicals; 543.65
• DOI: 10.1002/jms.3426
• Languages: English
• ISSNs: 1076-5174

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Mass spectrometry (MS) profiling techniques are used for analysing metabolites and xenobiotics in biofluids; however, detection of low abundance compounds using conventional MS techniques is poor. To counter this, nanoflow ultra‐high‐pressure liquid chromatography‐nanoelectrospray ionization‐time‐of‐flight MS (nUHPLC‐nESI‐TOFMS), which has been used primarily for proteomics, offers an innovative prospect for profiling small molecules. Compared to conventional UHPLC‐ESI‐TOFMS, nUHPLC‐nESI‐TOFMS enhanced detection limits of a variety of (xeno)metabolites by between 2 and 2000‐fold. In addition, this study demonstrates for the first time excellent repeatability and reproducibility for analysis of urine and plasma samples using nUHPLC‐nESI‐TOFMS, supporting implementation of this platform as a novel approach for high‐throughput (xeno)metabolomics. Copyright © 2014 John Wiley &amp; Sons, Ltd.</p> </abstract>