In vitro evaluation of human fetal osteoblast response to magnesium loaded mesoporous TiO2 coating. Issue 11 (20th December 2013)

• Record Type: Journal Article
• Authors: Cecchinato, Francesca; Xue, Ying; Karlsson, Johan; He, Wenxiao; Wennerberg, Ann; Mustafa, Kamal; Andersson, Martin; Jimbo, Ryo
• Is Part Of: Journal of biomedical materials research. Volume 102:Issue 11(2014)
• Page Start: 3862
• Page End: 3871
• Publication Date: 2013-12-20
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.a.35062
• Languages: English
• ISSNs: 1549-3296

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>This work aimed to evaluate the <italic>in vitro</italic> response of Transfected Human Foetal Osteoblast (hFOB) cultured on a magnesium‐loaded mesoporous TiO<sub>2</sub> coating. The application of mesoporous films on titanium implant surfaces has shown very promising potential to enhance osseointegration. This type of coating has the ability to act as a framework to sustain bioactive agents and different drugs. Magnesium is the element that, after calcium, is the most frequently used to dope titanium implant surfaces, since it is crucial for protein formation, growth factor expression, and aids for bone mineral deposition on implant surfaces. Mesoporous TiO<sub>2</sub> films with an average pore‐size of 6 nm were produced by the evaporation‐induced self‐assembly method (EISA) and deposited onto titanium discs. Magnesium loading was performed by soaking the mesoporous TiO<sub>2</sub> discs in a magnesium chloride solution. Surface characterization was conducted by SEM, XPS, optical interferometry, and AFM. Magnesium release profile was assessed at different time points using a Magnesium Detection kit. Cell morphology and spreading were observed with SEM. The cytoskeletal organization was stained with TRITC‐conjugated Phalloidin and cell viability was evaluated through a mitochondrial colorimetric (MTT) assay. Furthermore, gene expression of bone markers and cell mineralization were analyzed by real time RT‐PCR and alizarin‐red staining, respectively. The surface chemical analysis by XPS revealed the successful adsorption of magnesium to the mesoporous coating. The AFM measurements revealed the presence of a nanostructured surface roughness. Osteoblasts viability and adhesion as well as the gene expression were unaffected by the addition of magnesium possibly due to its rapid burst release, however, were enhanced by the 3D nanostructure of the TiO<sub>2</sub> layer. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3862–3871, 2014.</p> </abstract>