Fourier transform infrared microspectroscopy reveals unique phenotypes for human embryonic and induced pluripotent stem cell lines and their progeny. Issue 10 (25th April 2013)

• Record Type: Journal Article
• Authors: Cao, Julie; Ng, Elizabeth S.; McNaughton, Don; Stanley, Edouard G.; Elefanty, Andrew G.; Tobin, Mark J.; Heraud, Philip
• Is Part Of: Journal of biophotonics. Volume 7:Issue 10(2014)
• Page Start: 767
• Page End: 781
• Publication Date: 2013-04-25
• Subjects: Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201200217
• Languages: English
• ISSNs: 1864-063X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Fourier transform infrared (FTIR) microspectroscopy was employed to elucidate the macromolecular phenotype of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) and their differentiated progeny. Undifferentiated hESCs and hiPSC lines were found to be not clearly distinguishable from each other. However, although both hESC and hiPSC variants appeared to undergo similar changes during differentiation in terms of cell surface antigens, the derived cell types from all cell lines could be discriminated using FTIR spectroscopy. We foresee a possible future role for FTIR microspectroscopy as a powerful and objective investigative and quality control tool in regenerative medicine. (© 2014 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)</p> </abstract>