Comparison of two real‐time quantitative polymerase chain reaction strategies for minimal residual disease evaluation in lymphoproliferative disorders: correlation between immunoglobulin gene mutation load and real‐time quantitative polymerase chain reaction performance†. Issue 3 (19th November 2013)

• Record Type: Journal Article
• Authors: Della Starza, Irene; Cavalli, Marzia; Del Giudice, Ilaria; Barbero, Daniela; Mantoan, Barbara; Genuardi, Elisa; Urbano, Marina; Mannu, Claudia; Gazzola, Anna; Ciabatti, Elena; Guarini, Anna; Foà, Robin; Galimberti, Sara; Piccaluga, Pierpaolo; Gaidano, Gianluca; Ladetto, Marco; Monitillo, Luigia
• Is Part Of: Hematological oncology. Volume 32:Issue 3(2014:Sep.)
• Page Start: 133
• Page End: 138
• Publication Date: 2013-11-19
• Subjects: Hematological oncology -- Periodicals; Hematology; Medical Oncology; 616.99418005
• DOI: 10.1002/hon.2095
• Languages: English
• ISSNs: 0278-0232

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>We compared two strategies for minimal residual disease evaluation of B‐cell lymphoproliferative disorders characterized by a variable immunoglobulin heavy chain (<italic>IGH</italic>) genes mutation load. Twenty‐five samples from chronic lymphocytic leukaemia (<italic>n</italic> = 18) or mantle cell lymphoma (<italic>n</italic> = 7) patients were analyzed. Based on <italic>IGH</italic> variable region genes, 22/25 samples carried &gt;2% mutations, 20/25 &gt; 5%. In the <italic>IGH</italic> joining region genes, 23/25 samples carried &gt;2% mutations, 18/25 &gt; 5%. Real‐time quantitative polymerase chain reaction was performed on IGH genes using two strategies: method A utilizes two patient‐specific primers, whereas method B employs one patient‐specific and one germline primer, with different positions on the variable, diversity and joining regions. Twenty‐three samples (92%) resulted evaluable using method A, only six (24%) by method B. Method B poor performance was specifically evident among mutated <italic>IGH</italic> variable/joining region cases, although no specific mutation load above, which the real‐time quantitative polymerase chain reaction failed was found. The molecular strategies for minimal residual disease evaluation should be adapted to the B‐cell receptor features of the disease investigated. Copyright © 2013 John Wiley &amp; Sons, Ltd.</p> </abstract>