DNA‐Templated Biomimetic Enzyme Sheets on Carbon Nanotubes to Sensitively In Situ Detect Superoxide Anions Released from Cells. (17th July 2014)

• Record Type: Journal Article
• Authors: Ma, Xiaoqing; Hu, Weihua; Guo, Chunxian; Yu, Ling; Gao, Lixia; Xie, Jiale; Li, Chang Ming
• Is Part Of: Advanced functional materials. Volume 24:Number 37(2014)
• Page Start: 5897
• Page End: 5903
• Publication Date: 2014-07-17
• Subjects: Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.201401443
• Languages: English
• ISSNs: 1616-301X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Superoxide anion (O<sub>2</sub><sup>−</sup>) is implicated in a wide variety of biological phenomena and oxidative stress‐related diseases. The electrochemical detection of O<sub>2</sub><sup>−</sup> is very attractive but relies on superoxide dismutase enzymes, thus suffering from high cost and low durability. The advances of nanoscience allows architecting while functionalizing a biomimetic sensing platform in nanoscales for high sensitivity and specificity. In this work, manganous phosphate (Mn<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>) nanosheets, a biomimetic enzyme, are template‐synthesized with DNA and further assembled on carbon nanotubes (CNTs) to form unique DNA‐Mn<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>‐CNT nanocomposite sheets, of which the Mn<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub> sheets efficiently catalyze the dismutation of O<sub>2</sub><sup>−</sup> while CNTs enable fast electron transfer, thus achieving highly sensitive and specific detection of O<sub>2</sub><sup>−</sup> with long‐term stability. The biomimetic O<sub>2</sub><sup>−</sup> sensor is further used to monitor O<sub>2</sub><sup>−</sup><italic>in situ</italic> released from mouse cancer cell and normal skin cell under drug stimulation, showing excellent real time quantitative detection capability. This work demonstrates a nanoscale approach to not only synthesize but also design a biomimetic enzyme for comparable performance with the natural enzyme‐based biosensor while rendering much higher durability than the natural one and thus holding a great promise for broad applications in fundamental research, clinic diagnostics and screening for drug therapy effects.</p> </abstract>