Aqueous and lipid nuclear magnetic resonance metabolomic profiles of the earthworm Aporrectodea caliginosa show potential as an indicator species for environmental metabolomics. (22nd August 2014)

• Record Type: Journal Article
• Authors: Brown, Jeffrey N.; Samuelsson, Linda; Bernardi, Giuliana; Gooneratne, Ravi; Larsson, D.G. Joakim
• Is Part Of: Environmental toxicology and chemistry. Volume 33:Number 10(2014:Oct.)
• Page Start: 2313
• Page End: 2322
• Publication Date: 2014-08-22
• Subjects: Pollution -- Environmental aspects -- Periodicals; Environmental chemistry -- Periodicals; 615.902
• DOI: 10.1002/etc.2680
• Languages: English
• ISSNs: 0730-7268

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="etc2680-sec-0001" sec-type="section"> <p>The common pasture earthworm <italic>Aporrectodea caliginosa</italic> has often been neglected in environmental metabolomics in favor of species easily bred in the laboratory. The present study assigns aqueous metabolites in <italic>A. caliginosa</italic> using high‐resolution 1‐ and 2‐dimensional nuclear magnetic resonance (NMR) spectroscopy. In total, 51 aqueous metabolites were identified, including typical amino acids (alanine, leucine, asparagine, phenylalanine), sugars (maltose, glucose), the dominant earthworm‐specific 2‐hexyl‐5‐ethyl‐furansulfonate, and several previously unreported metabolites (oxoglutarate, putrescine). Examining the lesser‐known earthworm lipid metabolome showed various lipid fatty acyl chains, cholesterol, and phosphatidylcholine. To briefly test if the NMR metabolomic techniques could differentiate <italic>A. caliginosa</italic> from different sites, earthworms were collected from 2 adjacent farms. Orthogonal partial least squares discriminant analysis detected metabolomic differences, suggesting the worms from the 2 sites differed in their energy metabolism, as indicated by altered levels of alanine, glutamine, glutamate, malate, fumarate, and lipids. Evidence of greater utilization of lipid energy reserves and onset of protein catabolism was also present. While the precise cause of the metabolomic differences could not be determined, the results show the potential of this species for further environmental metabolomic studies. <italic>Environ Toxicol Chem 2014;33:2313–2322</italic>. © 2014 SETAC</p> </sec> </abstract>