Toward enantioselective nano ultrahigh‐performance liquid chromatography with Whelk‐O1 chiral stationary phase. Issue 19 (14th August 2014)

• Record Type: Journal Article
• Authors: Ciogli, Alessia; Pierri, Giuseppe; Kotoni, Dorina; Cavazzini, Alberto; Botta, Lorenzo; Villani, Claudio; Kocergin, Jelena; Gasparrini, Francesco; Chankvetadze, Bezhan
• Is Part Of: Electrophoresis. Volume 35:Issue 19(2014:Oct.)
• Page Start: 2819
• Page End: 2823
• Publication Date: 2014-08-14
• Subjects: Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201400240
• Languages: English
• ISSNs: 0173-0835

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>In this study, a Whelk‐O1 chiral stationary phase immobilized on 2.5 μm silica particles was employed in nanoLC. Two nanocolumns (180 and 250 mm long, 75 μm id) with a single polymeric organic monolithic outlet frit were packed under high‐pressure ultrasonic‐assisted packing procedure. The monolithic outlet frit was prepared by thermal polymerization of methacrylate‐based monomers affording high‐mechanical stability and high‐pressure resistance. Very efficient enantioseparations with more than 70 000 plates/m were achieved in normal phase mode by eluting (+/−) acenaphthenol. Nanocolumns were also tested in RP mode by using on‐line MS detection with nano‐spray ESI ion source. Kinetic performances of columns in RP mode were comparable to those in normal phase‐conditions.</p> </abstract>