Subplate in a rat model of preterm hypoxia–ischemia. (September 2014)

• Record Type: Journal Article
• Authors: Okusa, Chika; Oeschger, Franziska; Ginet, Vanessa; Wang, Wei‐Zhi; Hoerder‐Suabedissen, Anna; Matsuyama, Tomohiro; Truttmann, Anita C.; Molnár, Zoltán
• Is Part Of: Annals of clinical and translational neurology. Volume 1:Number 9(2014)
• Page Start: 679
• Page End: 691
• Publication Date: 2014-09
• Subjects: Nervous system -- Diseases -- Periodicals; Neurology -- Periodicals; 616.8005
• DOI: 10.1002/acn3.97
• Languages: English
• ISSNs: 2328-9503

Abstract

<abstract abstract-type="main" id="acn397-abs-0001"> <title>Abstract</title> <sec id="acn397-sec-0001" sec-type="section"> <title>Objective</title> <p>Hypoxia–ischemia (HI) in preterm infants primarily leads to injuries in the cerebral white matter. However, there is growing evidence that perinatal injury in preterms can also involve other zones including the cortical gray matter. In a neonatal rat model of HI, selective vulnerability of subplate has been suggested using BrdU birth‐dating methods. In this study, we aimed to investigate the neuropathological changes of the subplate and deep layers of the cortex following cerebral HI in neonatal rats with specific cell markers.</p> </sec> <sec id="acn397-sec-0002" sec-type="section"> <title>Methods</title> <p>P2 rats underwent permanent occlusion of the right common carotid artery followed by a period of hypoxia. P8 rats were analyzed using immunohistochemistry; subplate and deep layers cells were quantified and compared with sham‐operated case.</p> </sec> <sec id="acn397-sec-0003" sec-type="section"> <title>Results</title> <p>A large variability in the extent of the cerebral injury was apparent. For the three analyzed subplate populations (Nurr1+, Cplx3+, and Ctgf+ cells), no significant cell reduction was observed in mild and moderate cases. Only in severe cases, subplate cells were strongly affected, but these injuries were always accompanied by the cell reductions in layers VI and V.</p> </sec> <sec id="acn397-sec-0004" sec-type="section"> <title>Interpretation</title> <p>We could therefore not confirm a specific vulnerability of subplate cells compared to other deep layers or the white matter in our model.</p> </sec> </abstract>