Synthesis of Mono‐ and Dihydroxylated Amino Acids with New α‐Ketoglutarate‐Dependent Dioxygenases: Biocatalytic Oxidation of CH Bonds. Issue 10 (11th September 2014)

• Record Type: Journal Article
• Authors: Baud, Damien; Saaidi, Pierre‐Loïc; Monfleur, Adam; Harari, Marine; Cuccaro, Julien; Fossey, Aurélie; Besnard, Marielle; Debard, Adrien; Mariage, Aline; Pellouin, Virginie; Petit, Jean‐Louis; Salanoubat, Marcel; Weissenbach, Jean; de Berardinis, Véronique; Zaparucha, Anne
• Is Part Of: ChemCatChem. Volume 6:Issue 10(2014:Oct.)
• Page Start: 3012
• Page End: 3017
• Publication Date: 2014-09-11
• Subjects: Catalysis -- Periodicals; 541.39505
• DOI: 10.1002/cctc.201402498
• Languages: English
• ISSNs: 1867-3880

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Iron(II)/α‐ketoacid‐dependent oxygenases (αKAOs) are enzymes that mainly catalyse hydroxylation reaction. By using a genomic approach combining sequence comparison and protein‐domain sharing, a set of 131 αKAO enzymes was prepared. The screening of various substrates revealed five new αKAOs. Four αKAOs were found to be active towards <sc>L</sc>‐lysine, <sc>L</sc>‐ornithine and <sc>L</sc>‐arginine with total regio‐ and stereoselectivities and yielding the corresponding 3‐ or 4‐hydroxyamino acids. The enzymatic cascade reaction with two stereoselective regiodivergent αKAOs enabled the synthesis of 3, 4‐dihydroxy‐<sc>L</sc>‐lysine.</p> </abstract>