Chemical and Genetic Studies on Hybrid of Ligularia subspicata and Ligularia cyathiceps Collected in Yunnan Province of China. Issue 9 (September 2014)

• Record Type: Journal Article
• Authors: Shimizu, Anna; Watanabe, Shizuka; Hanai, Ryo; Okamoto, Yasuko; Tori, Motoo; Gong, Xun; Kuroda, Chiaki
• Is Part Of: Chemistry & biodiversity. Volume 11:Issue 9(2014:Sep.)
• Page Start: 1438
• Page End: 1448
• Publication Date: 2014-09
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Biodiversity -- Periodicals; 572
• DOI: 10.1002/cbdv.201400133
• Languages: English
• ISSNs: 1612-1872

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Two morphologically ambiguous <italic>Ligularia</italic> samples (samples <italic>A</italic> and <italic>B</italic>), and samples with morphology of <italic>Ligularia subspicata</italic> (sample <italic>C</italic>), <italic>Ligularia lamarum</italic> (sample <italic>D</italic>), or <italic>Ligularia cyathiceps</italic> (sample <italic>E</italic>), were collected at Tianchi Pond, Shangrila County, Yunnan Province, China. Analysis of the nucleotide sequence of the internal transcribed spacers (ITSs) in the nuclear ribosomal RNA gene cluster indicated that not only sample <italic>B</italic> but also sample <italic>D</italic> was a hybrid of <italic>L. cyathiceps</italic> and <italic>L. lamarum</italic>/<italic>L. subspicata.</italic> Although the morphology of sample <italic>A</italic> suggested that it was also a hybrid, the ITS sequence of sample <italic>A</italic> was that of <italic>L. cyathiceps.</italic> Twenty compounds were isolated from the five samples, and the structures of two new compounds <bold>7</bold> and <bold>14</bold> were determined. Furanoeremophilanes typical of <italic>L. lamarum</italic>/<italic>L. subspicata</italic> were detected not only in samples <italic>C</italic> and <italic>D</italic>, but also in samples <italic>A</italic> and <italic>B.</italic> These results indicate that the ability to produce root chemicals can spread through introgression.</p> </abstract>