Hemoglobin regulates the migration of glioma cells along poly(ε‐caprolactone)‐aligned nanofibers. (24th July 2014)

• Record Type: Journal Article
• Authors: Roth, Alexander D.; Elmer, Jacob; Harris, David R.; Huntley, Joseph; Palmer, Andre F.; Nelson, Tyler; Johnson, Jed K.; Xue, Ruipeng; Lannutti, John J.; Viapiano, Mariano S.
• Is Part Of: Biotechnology progress. Volume 30:Number 5(2014)
• Page Start: 1214
• Page End: 1220
• Publication Date: 2014-07-24
• Subjects: Biotechnology -- Periodicals; Food industry and trade -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/btpr.1950
• Languages: English
• ISSNs: 8756-7938

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Aligned fibers have been shown to facilitate cell migration in the direction of fiber alignment while oxygen (O<sub>2</sub>)‐carrying solutions improve the metabolism of cells in hypoxic culture. Therefore, U251 aggregate migration on poly(ε‐caprolactone) (PCL)‐aligned fibers was studied in cell culture media supplemented with the O<sub>2</sub> storage and transport protein hemoglobin (Hb) obtained from bovine, earthworm and human sources at concentrations ranging from 0 to 5 g/L within a cell culture incubator exposed to O<sub>2</sub> tensions ranging from 1 to 19% O<sub>2</sub>. Individual cell migration was quantified using a wound healing assay. In addition, U251 cell aggregates were developed and aggregate dispersion/cell migration quantified on PCL‐aligned fibers. The results of this work show that the presence of bovine or earthworm Hb improved individual cell viability at 1% O<sub>2</sub>, while human Hb adversely affected cell viability at increasing Hb concentrations and decreasing O<sub>2</sub> levels. The control data suggests that decreasing the O<sub>2</sub> tension in the incubator from 5 to 1% O<sub>2</sub> decreased aggregate dispersion on the PCL‐aligned fibers. However, the addition of bovine Hb at 5% O<sub>2</sub> significantly improved aggregate dispersion. At 19% O<sub>2</sub>, Hb did not impact aggregate dispersion. Also at 1% O<sub>2</sub>, aggregate dispersion appeared to increase in the presence of earthworm Hb, but only at the latter time points. Taken together, these results show that Hb‐based O<sub>2</sub> carriers can be utilized to improve O<sub>2</sub> availability and the migration of glioma spheroids on nanofibers. © 2014 American Institute of Chemical Engineers <italic>Biotechnol. Prog</italic>., 30:1214–1220, 2014</p> </abstract>