Rapid molecular screening for multidrug‐resistant tuberculosis in a resource‐limited region of China. Issue 10 (17th July 2014)

• Record Type: Journal Article
• Authors: Zhang, Dan; Liu, Beizhong; Wang, Yufeng; Pang, Yu
• Is Part Of: Tropical medicine & international health. Volume 19:Issue 10(2014:Oct.)
• Page Start: 1259
• Page End: 1266
• Publication Date: 2014-07-17
• Subjects: Tropical medicine -- Periodicals; Public health -- Periodicals; 616.988
• DOI: 10.1111/tmi.12359
• Languages: English
• ISSNs: 1360-2276

Abstract

<abstract abstract-type="main" id="tmi12359-abs-0001"> <title>Abstract</title> <sec id="tmi12359-sec-0001" sec-type="section"> <title>Objective</title> <p>To investigate the molecular characteristics of MDR and XDR strains circulating in Chongqing, China.</p> </sec> <sec id="tmi12359-sec-0002" sec-type="section"> <title>Methods</title> <p>The drug target genes conferring for rifampicin (RIF), isoniazid (INH), ethambutol (EMB), ofloxacin (OFLX) and kanamycin (KAN) resistance were screened by DNA sequencing to determine the mutation frequencies in this area.</p> </sec> <sec id="tmi12359-sec-0003" sec-type="section"> <title>Results</title> <p>Drug susceptibility of 208 MDR isolates revealed that 132 (63.46%) were resistant to streptomycin (SM), 96 (46.15%) to ethambutol (EMB), 51 (24.52%) to ofloxacin (OFLX), and 26 (12.50%) to kanamycin (KAN); six (2.88%) isolates had XDR profiles. In comparison with the drug susceptibility phenotype, the sensitivity of drug resistance by DNA sequencing was 91.83% for RIF, 87.50% for INH, 66.67% for EMB, 74.51% for OFLX and 53.85% for KAN resistance. 12.50% of EMB‐ and 1.27% of OFLX‐susceptible isolates were harboured genetic mutations in <italic>embB</italic> and <italic>gyrA</italic>, respectively.</p> </sec> <sec id="tmi12359-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Our findings demonstrate that the hot‐spot regions localised in <italic>rpoB</italic>, <italic> katG</italic> and <italic>inhA</italic> genes serve as excellent markers for the corresponding drug resistance, while EMB, OFLX or KAN drug‐resistant TB cases may not be identifiable by scanning <italic>embB</italic>, <italic> gyrA, rrs</italic> and <italic>eis promoter</italic> in Chongqing, indicating that further studies on the drug resistance mechanisms of EMB, OFLX and KAN are urgently needed to elucidate the low sensitivity between genomic substitutions and drug‐resistant phenotype.</p> </sec> </abstract>