Wuchereria bancrofti 20/22 a homologue of abundant larval transcript L3 stage filarial antigen: molecular and immunological characterization. (October 2014)

• Record Type: Journal Article
• Authors: Aparnaa, Ramanathan; Mahalakshmi, Natarajan; Harini, Asai; Jeyaprita, Parasurama Jawaharlal; Anugraha, Gandhirajan; Amdare, N. P.; Khatri, V. K.; Reddy, M. V. R.; Kaliraj, P.
• Is Part Of: Parasite immunology. Volume 36:Number 10(2014:Oct.)
• Page Start: 475
• Page End: 484
• Publication Date: 2014-10
• Subjects: Veterinary parasitology -- Immunological aspects -- Periodicals; Host-parasite relationships -- Immunological aspects -- Periodicals; 571.96
• DOI: 10.1111/pim.12120
• Languages: English
• ISSNs: 0141-9838

Abstract

<abstract abstract-type="main" id="pim12120-abs-0001"> <title>Summary</title> <p>The chromadorea abundant larval transcript (ALT) family of proteins contains ALT one of the most studied putative vaccine candidate in experimental filariasis. This study reports the characterization of <italic>Wuchereria bancrofti</italic> 20/22 (Wb20/22) as a member of chromadorea, the ALT family of proteins from the L3 stage of <italic>W. bancrofti</italic>. The high reactivity with serum from the endemic normal (EN) population suggests that Wb20/22 could be a target of elicit protective immunity. The glutamic acid‐rich region of Wb20/22 was predicted to harbour the longest linear B‐cell epitope by insilico prediction tools. The significance of this region was revealed by studying the mutant form of Wb20/22, without acidic domain (WOAD) which was cloned, and the immune response was compared with Wb20/22. The signal sequence of Wb20/22 was also an immunodominant region, and mutant construct without signal sequence (WOSS) was cloned and characterized. The peak antibody titre elicited by WOAD was higher than Wb20/22 or WOSS, which pointed to the immunomodulatory role of glutamic acid‐rich region. Wb20/22 elicited very high levels of IL‐10 and diminished levels of IL‐4 and IL‐5 which could be the reason for low antibody titre. The prophylactic efficacy of WOAD conferred protection (62·26%) which was higher than Wb20/22 (49·82%) and WOSS (54·78%).</p> </abstract>