Novel and rapid enumeration method of peripheral blood stem cells using automated hematology analyzer. (27th December 2013)

• Record Type: Journal Article
• Authors: Tanosaki, R.; Kumazawa, T.; Yoshida, A.; Oguni, S.; Nakano, A.; Yamagata, S.; Takahashi, N.; Kurosawa, S.; Kim, S. W.; Yamashita, T.; Mori, S.; Heike, Y.; Fukuda, T.; Hamaguchi, Y.; Tsuda, H.
• Is Part Of: International journal of laboratory hematology. Volume 36:Number 5(2014:Oct.)
• Page Start: 521
• Page End: 530
• Publication Date: 2013-12-27
• Subjects: Hematology -- Periodicals; Blood -- Diseases -- Periodicals; Hematology -- Periodicals; 616.15005
• DOI: 10.1111/ijlh.12182
• Languages: English
• ISSNs: 1751-5521

Abstract

<abstract abstract-type="main" id="ijlh12182-abs-0001"> <title>Summary</title> <sec id="ijlh12182-sec-0001" sec-type="section"> <title>Introduction</title> <p>The number of infused CD34<sup>+</sup> cells is crucial to the success of peripheral blood stem cell transplantation (PBSCT). Here, we present, for the first time, a new method of enumerating hematopoietic progenitor cells (HPCs) for PBSCT.</p> </sec> <sec id="ijlh12182-sec-0002" sec-type="section"> <title>Method</title> <p>This novel method is based on hemolysis and chemical staining, followed by flow cytometry‐based optical detection, conducted using an automated hematology analyzer (XN series, Sysmex). CD34<sup>+</sup> cells and HPCs were compared in 76 granulocyte colony‐stimulating factor (G‐CSF)‐mobilized blood or apheresis samples taken from healthy donors (<italic>n</italic> = 18) or patients undergoing autologous PBSCT (<italic>n</italic> = 6).</p> </sec> <sec id="ijlh12182-sec-0003" sec-type="section"> <title>Results</title> <p>There was a strong correlation between the numbers of HPCs and CD34<sup>+</sup> cells (<italic>R</italic><sup>2</sup> = 0.958). The expected total number of HPCs in the final products, which was estimated from HPCs in pre‐apheresis PB or mid‐apheresis products, also correlated well with the total number of CD34<sup>+</sup> cells in the final products. The change in HPCs in PB closely resembled that of CD34<sup>+</sup> cells during mobilization. Experiments using immunomagnetic beads suggested that the majority of CD34<sup>+</sup> cells existed in HPCs, and vice versa.</p> </sec> <sec id="ijlh12182-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Hematopoietic progenitor cells may serve as surrogates for CD34<sup>+</sup> cells in PBSCT. However, further investigations are required to verify this.</p> </sec> </abstract>