Amplification of 3q26 and 5p15 regions in cervical intraepithelial neoplasia. (17th September 2014)

• Record Type: Journal Article
• Authors: Kudela, Erik; Farkasova, Anna; Visnovsky, Jozef; Balharek, Tomas; Sumichrastova, Petra; Sivakova, Jana; Plank, Lukas; Danko, Jan
• Is Part Of: Acta obstetricia et gynecologica Scandinavica. Volume 93:Number 10(2014)
• Page Start: 997
• Page End: 1002
• Publication Date: 2014-09-17
• Subjects: Gynecology -- Periodicals; Pregnancy -- Periodicals; Obstetrics -- Periodicals; 618.05
• DOI: 10.1111/aogs.12485
• Languages: English
• ISSNs: 0001-6349

Abstract

<abstract abstract-type="main" id="aogs12485-abs-0001"> <title>Abstract</title> <sec id="aogs12485-sec-0001" sec-type="section"> <title>Objective</title> <p>To analyze different amplification patterns of 3q26 and 5p15 regions in low‐grade and high‐grade cervical intraepithelial neoplasia.</p> </sec> <sec id="aogs12485-sec-0002" sec-type="section"> <title>Design</title> <p>Experimental research.</p> </sec> <sec id="aogs12485-sec-0003" sec-type="section"> <title>Setting</title> <p>Department of Obstetrics and Gynecology at a medical faculty in Slovakia.</p> </sec> <sec id="aogs12485-sec-0004" sec-type="section"> <title>Population</title> <p>A group of 83 patients referred for colposcopic examination.</p> </sec> <sec id="aogs12485-sec-0005" sec-type="section"> <title>Methods</title> <p>Amplification of 3q26 and 5p15 regions was analyzed on the 100 most atypical cells from a cervical cytology slide by fluorescent in situ hybridization using a multicolor hybridization probe. Chi‐squared and Man–Whitney <italic>U</italic>‐tests were used for statistical analysis.</p> </sec> <sec id="aogs12485-sec-0006" sec-type="section"> <title>Main outcome measures</title> <p>Liquid‐based cytology samples and biopsy samples obtained during colposcopic examination correlated with high‐risk human papillomavirus status and with amplification patterns of selected regions analyzed by fluorescent in situ hybridization.</p> </sec> <sec id="aogs12485-sec-0007" sec-type="section"> <title>Results</title> <p>The number of cells with 3q26 and 5p15 gain rises with the severity of the lesion <italic>p </italic>< 0.01. The sensitivity of 3q26 amplification for CIN2+ lesions was 72.1% (95% confidence interval 56.3–84.7) and specificity was 90.0% (95% confidence interval 76.3–97.1). The sensitivity of 5p15 amplification for CIN2+ lesions was 69.8% (95% confidence interval 53.9–82.8) and specificity was 85.0% (95% confidence interval 70.2–94.3).</p> </sec> <sec id="aogs12485-sec-0008" sec-type="section"> <title>Conclusion</title> <p>Evaluation of telomerase components can help in differential diagnosis of low‐grade and high‐grade cervical lesions and in individualized management of these patients.</p> </sec> </abstract>