Generation and characterization of Lhx9‐GFPCreERT2 knock‐in mouse line. Issue 9 (12th August 2014)

• Record Type: Journal Article
• Authors: Balasubramanian, Revathi; Bui, Andrew; Xie, Xiaoling; Deng, Min; Gan, Lin
• Is Part Of: Genesis. Volume 52:Issue 9(2014:Sep.)
• Page Start: 827
• Page End: 832
• Publication Date: 2014-08-12
• Subjects: Developmental genetics -- Periodicals; Genetics -- Periodicals; Developmental biology -- Periodicals; Embryology -- Periodicals; Genetic regulation -- Periodicals; 576.5
• DOI: 10.1002/dvg.22805
• Languages: English
• ISSNs: 1526-954X

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p>LHX9 is a LIM‐homeodomain transcription factor essential for the development of gonads, spinal cord interneurons, and thalamic neurons to name a few. We recently reported the expression of LHX9 in retinal amacrine cells during development. In this study, we generated an <italic>Lhx9‐GFPCreER^T²</italic> (GCE) knock‐in mouse line by knocking‐in a GCE cassette at the <italic>Lhx9</italic> locus, thus inactivating endogenous <italic>Lhx9</italic>. <italic>Lhx9^GCE^/+</italic> mice were viable, fertile, and displayed no overt phenotypical characteristics. <italic>Lhx9^GCE^/^GCE</italic> mice were all phenotypically female, smaller in size, viable, but infertile. The specificity and efficacy of the <italic>Lhx9‐GCE</italic> mouse line was verified by crossing it to a <italic>Rosa26‐tdTomato</italic> reporter mouse line, which reveals the Cre recombinase activities in retinal amacrine cells, developing limbs, testis, hippocampal neurons, thalamic neurons, and cerebellar neurons. Taken together, the <italic>Lhx9‐GCE</italic> mouse line could serve as a beneficial tool for lineage tracing and gene manipulation experiments. genesis 52:827–832, 2014. © 2014 Wiley Periodicals, Inc.</p> </abstract>