Chemical profiling and in vitro biological effects of Cardiospermum halicacabum L. (Sapindaceae) aerial parts and seeds for applications in neurodegenerative disorders. (October 2014)

• Record Type: Journal Article
• Authors: Menichini, Francesco; Losi, Luigi; Bonesi, Marco; Pugliese, Alessandro; Loizzo, Monica R.; Tundis, Rosa
• Is Part Of: Journal of enzyme inhibition and medicinal chemistry. Volume 29:Number 5(2014:Oct.)
• Page Start: 677
• Page End: 685
• Publication Date: 2014-10
• Subjects: Enzyme inhibitors -- Periodicals; Enzyme Inhibitors -- periodicals; Biochemistry -- periodicals; 572.7
• DOI: 10.3109/14756366.2013.840614
• Languages: English
• ISSNs: 1475-6366

Abstract

<abstract> <title>Abstract</title> <p> <italic>Cardiospermum halicacabum</italic> is widely used in traditional medicine. Previous studies have focused on the aerial parts, while the seeds have been poorly investigated. This work aimed to analyse the chemical composition of extracts from aerial parts and seeds obtained using Naviglio and Soxhlet (PN, PS, and SN, SS, respectively), the inhibitory properties against tyrosinase, acetylcholinesterase (AChE), and butyrylcholinesterase (BChE) and the antioxidant effects. PN total extract showed significant anti-tyrosinase activity (IC₅₀ value of 10.8 µg/mL). After partitioning with <italic>n</italic>-hexane, an HPLC method for analysing chemical constituents was established. Apigenin, luteolin, and apigenin-7-<italic>O</italic>-glucoside are the predominant constituents. SN <italic>n</italic>-hexane fraction was the most active inhibitor of BChE (IC₅₀ of 57.9 µg/mL). Gas chromatography-mass spectrometry analysis revealed fatty acids, including eicosanoic acid, methyl 11-eicosenoate and oleic acid, as the major constituents. These findings suggest the potentiality of both seeds and aerial parts of <italic>C. halicacabum</italic> in the treatment of neurological disorders.</p> </abstract>