A novel GPR30 rs10235056 A>G polymorphism associated with post-transcriptional regulation in lymphoblastoid cell lines. (August 2014)

• Record Type: Journal Article
• Authors: Zhang, Wenwen; Yang, Fang; Luo, Jiayan; Chen, Fengxia; Gu, Jun; Guan, Xiaoxiang
• Is Part Of: Biomarkers. Volume 19:Number 5(2014)
• Page Start: 417
• Page End: 423
• Publication Date: 2014-08
• Subjects: Biochemical markers -- Periodicals; 610.28
• DOI: 10.3109/1354750X.2014.924996
• Languages: English
• ISSNs: 1354-750X

Abstract

<abstract> <title>Abstract</title> <p>Considering the role of variants in the 3′ untranslated region (3′UTR) of <italic>GPR30</italic> gene remains unclear, we analyzed the association between the variants at the <italic>GPR30</italic> gene 3′UTR miRNA binding sites and their mRNA expression using the data from the HapMap online database. Nine single nucleotide polymorphisms (SNPs) in <italic>GPR30</italic> gene 3′UTR had available minor allele frequency (MAF) values which were obtained. And the frequency distribution of all the selected <italic>GPR30</italic> gene 3′UTR variants genotypes among the different populations and pairwise linkage disequilibrium (LD) values were calculated. In addition, correlation analysis of the selected <italic>GPR30</italic> variants genotypes and their mRNA expression in the lymphoblastoid cell lines was performed, which showed that only rs10235056 was significantly associated with <italic>GPR30</italic> mRNA expression (<italic>p</italic> = 0.028), but rs4266553 (<italic>p</italic> = 0.304), rs3808353 (<italic>p</italic> = 0.900), rs3808354 (<italic>p</italic> = 0.739) and rs1133043 (<italic>p</italic> = 0.913) were insignificant. Taken together, the present study provides the first evidences that the GPR30 rs10235056 A &gt; G polymorphism could be a putative variant mediating its post-transcriptional regulation, which might support its use as markers of cancer risk and individualized treatment.</p> </abstract>