Attenuation of lung cancer stem cell tumorigenesis and metastasis by cisplatin. (1st October 2014)

• Record Type: Journal Article
• Authors: Wang, Shenxu; Ma, Sai; Li, Xiujuan; Xue, Zengfu; Zhang, Xiaotian; Fan, Weiwei; Nie, Yongzhan; Wu, Kaichun; Chen, Xiaoyuan; Cao, Feng
• Is Part Of: Experimental lung research. Volume 40:Number 8(2014)
• Page Start: 404
• Page End: 414
• Publication Date: 2014-10-01
• Subjects: Lungs -- Periodicals; Lungs -- Diseases -- Periodicals; Lung Diseases; Lung -- physiology; Respiratory System; 616.24
• DOI: 10.3109/01902148.2014.938201
• Languages: English
• ISSNs: 0190-2148

Abstract

<abstract> <title>ABSTRACT</title> <p> <italic>Purpose</italic>: To investigate the effect of cisplatin on the growth and metastasis abilities of lung cancer stem cells (CSCs) via molecular imaging. <italic>Materials and Methods</italic>: The expression changes of lung CSCs cell marker in A549-Luc-C8 human non-small-cell lung cancer (NSCLC) cell line with or without cisplatin treatment were detected by flow cytometry. The tumorigenesis and metastasis abilities of A549-Luc-C8 cells were monitored both in vitro and in vivo, and the mechanism was assessed by gene sequencing. <italic>Results</italic>: About 1%–2% of CSCs were detected in A549-Luc-C8 cells and decreased CSCs percentage was observed after cisplatin treatment. Attenuated tumorigenesis and metastasis abilities of A549-Luc-C8 cells were found in cisplatin treated group. <italic>Conclusions</italic>: Decreased percentage of CSCs in A549-Luc-C8 cells can be induced by cisplatin treatment, which may partly be attributed to the attenuated expression of growth factors.</p> </abstract>