Preliminary study of video imaging of blood vessels in tissues lining the gingival sulcus in periodontally healthy individuals. (11th December 2013)

• Record Type: Journal Article
• Authors: Townsend, D.; D'Aiuto, F.; Deanfield, J.
• Is Part Of: Journal of periodontal research. Volume 49:Number 5(2014:Oct.)
• Page Start: 670
• Page End: 679
• Publication Date: 2013-12-11
• Subjects: Periodontics -- Periodicals; 617.632
• DOI: 10.1111/jre.12150
• Languages: English
• ISSNs: 0022-3484

Abstract

<abstract abstract-type="main" id="jre12150-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jre12150-sec-0001" sec-type="section"> <title>Background and Objective</title> <p>The aim of this study was to obtain <italic>in vivo</italic> images of the microcirculation in tissues lining the gingival crevice in periodontally healthy volunteers and to assess the repeatability of the parameters measured.</p> </sec> <sec id="jre12150-sec-0002" sec-type="section"> <title>Material and Methods</title> <p>Video microscopy images of the microcirculation of tissues lining the gingival crevice were obtained from 20 periodontally healthy volunteers. Images were obtained with a single 1 mm diameter 1 pitch gradient index lens with a high numerical aperture and with a plain glass lens and illumination with a green 525 nm light‐emitting diode and recorded using a video microscope.</p> </sec> <sec id="jre12150-sec-0003" sec-type="section"> <title>Results</title> <p>The morphological features of the vessels (including vessel diameter, vessel density, loops, branches, dilated vessels) were similar to those described previously in other mammals. The Kappa values for the assessment of morphology of the vessels using the gradient index lens range from 0.83 for branching to 0.91 for dilated and using the glass lens 0.47 for branching and 0.38 for dilated.</p> </sec> <sec id="jre12150-sec-0004" sec-type="section"> <title>Conclusions</title> <p>This novel system allowed for a consistent and repeatable assessment of the gingival microvasculature. However, there was some evidence of possible pressure artefacts in those cases where the measurements of separation between vessels exceeded 150 μm.</p> </sec> </abstract>