Acute Vibration Induces Transient Expression of Anabolic Genes in the Murine Intervertebral Disc. Issue 7 (2nd July 2013)

• Record Type: Journal Article
• Authors: McCann, Matthew R.; Patel, Priya; Beaucage, Kim L.; Xiao, Yizhi; Bacher, Corey; Siqueira, Walter L.; Holdsworth, David W.; Dixon, S. Jeffrey; Séguin, Cheryle A.
• Is Part Of: Arthritis and rheumatism. Volume 65:Issue 7(2013:Jul.)
• Page Start: 1853
• Page End: 1864
• Publication Date: 2013-07-02
• Subjects: Arthritis -- Periodicals; Rheumatism -- Periodicals; Arthritis -- Periodicals; Rheumatic Diseases -- Periodicals; Rhumatisme -- Périodiques; Arthrite -- Périodiques; 616.72
• DOI: 10.1002/art.37979
• Languages: English
• ISSNs: 0004-3591

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="art37979-sec-0001" sec-type="section"> <title>Objective</title> <p>Low‐amplitude whole‐body vibration has been adopted for the treatment of back pain and spinal disorders. However, there is limited knowledge of the impact of vibration on the intervertebral disc (IVD). This study was undertaken to examine the effects of acute vibration on anabolic and catabolic pathways in the IVD and to characterize the dependence of these changes on time and frequency.</p> </sec> <sec id="art37979-sec-0002" sec-type="section"> <title>Methods</title> <p>Custom‐designed platforms were developed to apply acute vibration to ex vivo and in vivo mouse models. Spinal segments (ex vivo) or mice (in vivo) were subjected to vibration (for 30 minutes at 15–90 Hz with peak acceleration at 0.3<italic>g</italic>), and IVDs were examined at specific time points after vibration. Gene expression was quantified using real‐time polymerase chain reaction, and protein levels were examined by quantitative mass spectrometry and immunofluorescence.</p> </sec> <sec id="art37979-sec-0003" sec-type="section"> <title>Results</title> <p>In the ex vivo model, acute vibration at 15 Hz induced expression of anabolic genes (aggrecan, biglycan, decorin, type I collagen, and <italic>Sox9</italic>) and suppressed expression of <italic>Mmp13</italic>, with the most pronounced changes detected 6 hours following vibration. These beneficial effects were frequency dependent and were no longer evident between 45 and 90 Hz. In vivo, the effects on anabolic gene expression were even more robust and were accompanied by decreased expression of <italic>Adamts4, Adamts5, </italic> and <italic>Mmp3</italic>. Moreover, significant increases in the protein levels of aggrecan, biglycan, decorin, and type I collagen were detected in vivo.</p> </sec> <sec id="art37979-sec-0004" sec-type="section"> <title>Conclusion</title> <p>These findings demonstrate dramatic anabolic effects of acute vibration on IVD tissue, responses that are dependent on frequency. The similarity of the in vivo and ex vivo responses indicates that at least some effects of vibration are tissue autonomous.</p> </sec> </abstract>