Human dermal fibroblast migration induced by fibronectin in autocrine and paracrine manners. Issue 9 (September 2014)

• Record Type: Journal Article
• Authors: Li, Xiaoguang; Qian, Hua; Ono, Fumitake; Tsuchisaka, Atsunari; Krol, Rafal P.; Ohara, Koji; Hayakawa, Taihei; Matsueda, Satoko; Sasada, Tetsuro; Ohata, Chika; Furumura, Minao; Hamada, Takahiro; Hashimoto, Takashi
• Is Part Of: Experimental dermatology. Volume 23:Issue 9(2014:Sep.)
• Page Start: 682
• Page End: 684
• Publication Date: 2014-09
• Subjects: Dermatology -- Periodicals; 616.5
• DOI: 10.1111/exd.12447
• Languages: English
• ISSNs: 0906-6705

Abstract

<abstract abstract-type="main" id="exd12447-abs-0001"> <title>Abstract</title> <p>Although fibronectin (FN) is known as a chemoattractant for human dermal fibroblasts (HDFs), it is unclear whether HDF migration is stimulated by FN produced by HDFs (autocrine manner) or by keratinocytes (paracrine manner). In this study, we investigated HDF migration by Boyden chamber assay using conditioned media from HDFs and HaCaT cells (keratinocyte cell line). Immunoblotting and enzyme‐linked immunosorbent assay revealed that FN existed in both conditioned media. Boyden chamber assay showed both conditioned media stimulated HDF migration, which was inhibited by anti‐FN antibody. Antibodies to both integrin <italic>β</italic>1and <italic>β</italic>3 subunits inhibited HDF migration induced by HDF‐conditioned medium almost completely and that by HaCaT cell‐conditioned medium with 50–60%. These results suggested that HDF migration was stimulated by FN in both autocrine and paracrine manners. However, the mechanisms of HDF migration by FN, particularly the role of integrin <italic>β</italic>1 and <italic>β</italic>3 subunits, were slightly different between autocrine and paracrine manners.</p> </abstract>