The Anti‐Inflammatory Impact of Omega‐3 Polyunsaturated Fatty Acids During the Establishment of Endometriosis‐Like Lesions. (5th June 2014)

• Record Type: Journal Article
• Authors: Attaman, Jill A.; Stanic, Aleksandar K.; Kim, Minji; Lynch, Maureen P.; Rueda, Bo R.; Styer, Aaron K.
• Is Part Of: American journal of reproductive immunology. Volume 72:Number 4(2014:Oct.)
• Page Start: 392
• Page End: 402
• Publication Date: 2014-06-05
• Subjects: Human reproduction -- Immunological aspects -- Periodicals; 616.69206
• DOI: 10.1111/aji.12276
• Languages: English
• ISSNs: 1046-7408

Abstract

<abstract abstract-type="main" id="aji12276-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="aji12276-sec-0001" sec-type="section"> <title>Problem</title> <p>The anti‐inflammatory impact of three polyunsaturated fatty acids (3‐PUFA) in endometriosis is incompletely understood. The effect of 3‐PUFA on endometriosis‐like lesions is evaluated as a potential anti‐inflammatory treatment target.</p> </sec> <sec id="aji12276-sec-0002" sec-type="section"> <title>Method of Study</title> <p>Wild Type (WT) and transgenic <italic>Fat‐1</italic> mice (high levels of endogenous 3‐PUFA) were utilized in a uterine tissue transplant endometriosis model. Experimental donor × host pairs included: WT × WT (WW), WT × <italic>Fat‐1</italic> (WF), and <italic>Fat‐1</italic> × <italic>Fat‐1</italic> (FF). Cytokine content (IL‐1β, IL‐2, IL‐4, IL‐6, IL‐10, IL‐12, IL‐17A, IFN‐γ, TNF‐γ, MCP‐1 and RANTES) and immunocellular composition in lesions was determined.</p> </sec> <sec id="aji12276-sec-0003" sec-type="section"> <title>Results</title> <p>Intralesion IL‐6 in WF hosts was 99‐fold lower than WW hosts (<italic>P = </italic>0.03). Compared to WW host lesions, Cox‐2 levels were decreased in WF [1.5‐fold (<italic>P </italic>=<italic> </italic>0.02)] and FF [1.2‐fold (<italic>P = </italic>0.01)] host lesions, respectively, and intralesion VEGF expression was increased [1.8‐fold; <italic>P = </italic>0.02 (WF) and 1.5‐fold; <italic>P </italic>=<italic> </italic>0.01 (FF)]. Lesions in FF hosts demonstrated reduced phosphohistone 3 expression (70%; <italic>P </italic>=<italic> </italic>0.03) compared to WW control hosts.</p> </sec> <sec id="aji12276-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Systemic host 3‐PUFA levels influence immune, angiogenic, and proliferative factors implicated in the early establishment of endometriosis.</p> </sec> </abstract>