Novel Morphological Findings of Human Sperm Removal by Leukocytes in In Vivo and In Vitro Conditions: Preliminary Study. (26th June 2014)

• Record Type: Journal Article
• Authors: Piasecka, Malgorzata; Fraczek, Monika; Gaczarzewicz, Dariusz; Gill, Kamil; Szumala‐Kakol, Anna; Kazienko, Anna; Laszczynska, Maria; Lenart, Stanislaw; Beutin, Lothar; Kurpisz, Maciej
• Is Part Of: American journal of reproductive immunology. Volume 72:Number 4(2014:Oct.)
• Page Start: 348
• Page End: 358
• Publication Date: 2014-06-26
• Subjects: Human reproduction -- Immunological aspects -- Periodicals; 616.69206
• DOI: 10.1111/aji.12284
• Languages: English
• ISSNs: 1046-7408

Abstract

<abstract abstract-type="main" id="aji12284-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="aji12284-sec-0001" sec-type="section"> <title>Problem</title> <p>Biological significance of increased number of leukocytes in ejaculate remains a subject of controversy. The aim of this research was to describe the morphological characteristics of the sperm elimination by leukocytes in <italic>in vivo</italic> and <italic>in vitro</italic> conditions using natural stimulator of the immune system—uropathogenic <italic>Escherichia coli</italic>, O75:HNT, isolated from semen.</p> </sec> <sec id="aji12284-sec-0002" sec-type="section"> <title>Method of study</title> <p>The study was performed on ejaculated spermatozoa from normozoospermic men with leukocytospermia (<italic>n</italic> = 10, <italic>in vivo</italic> experiment) or without leukocytospermia (<italic>n</italic> = 15, <italic>in vitro</italic> experiment). Morphological observations were performed using light and scanning electron microscopy.</p> </sec> <sec id="aji12284-sec-0003" sec-type="section"> <title>Results</title> <p>Sperm removal by active leukocytes mediated by traditional phagocytosis and generation of extracellular traps were observed in <italic>in vivo</italic> and <italic>in vitro</italic> experiments.</p> </sec> <sec id="aji12284-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Our morphological data suggest that human germ cells activate leukocytes triggering both traditional phagocytosis and a novel trapping mechanism, followed by extensive sperm elimination.</p> </sec> </abstract>