Satellite tobacco mosaic virus refined to 1.4 Å resolution. (1st September 2014)

• Record Type: Journal Article
• Authors: Larson, Steven B.; Day, John S.; McPherson, Alexander
• Is Part Of: Acta crystallographica. Volume 70:Part 9(2014:Sep.)
• Page Start: 2316
• Page End: 2330
• Publication Date: 2014-09-01
• Subjects: Biomolecules -- Structure -- Periodicals; Physical biochemistry -- Periodicals; X-ray crystallography -- Periodicals; Crystallography -- Periodicals; 572
• DOI: 10.1107/S1399004714013789
• Languages: English
• ISSNs: 0907-4449

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Satellite tobacco mosaic virus</italic> (STMV) is among the smallest viruses, having 60 identical subunits arranged with <italic>T</italic> = 1 icosahedral symmetry. Its crystal structure was solved at 290 K and was refined using, in part, crystals grown in microgravity. Electron‐density maps revealed nearly 57% of the genomic ssRNA. Using six flash‐cooled crystals, diffraction data were recorded to 1.4 Å resolution and independent refinements of the STMV model were carried out <italic>versus</italic> the previous 1.8 Å resolution data representing merged data from 21 crystals (271 689 unique reflections), data consisting of corresponding reflections to 1.8 Å resolution from the cooled crystals and 1.4 Å resolution data from the cooled crystals comprised of 570 721 unique reflections. Models were independently refined with full NCS constraints using the program <italic>CNS</italic> and in restrained mode using the programs <italic>CNS</italic>, <italic>REFMAC</italic>5 and <italic>SHELX</italic>‐97, with the latter two procedures including anisotropic temperature factors. Significant additional structural detail emerged from the analyses, including a unique cation and anion arrangement on fivefold axes and a precise assessment of icosahedral symmetry exactness in the crystal lattice. STMV represents the highest resolution native virus structure currently known by a substantial margin, and it permits the evaluation of a precise atomic model of a spherical virus at near‐atomic resolution for the first time.</p> </abstract>