In Vitro Evaluation of Bacopa monniera Extract and Individual Constituents on Human Recombinant Monoamine Oxidase Enzymes. (21st January 2014)

• Record Type: Journal Article
• Authors: Singh, Rajbir; Ramakrishna, Rachumallu; Bhateria, Manisha; Bhatta, Rabi Sankar
• Is Part Of: Phytotherapy research. Volume 28:Number 9(2014:Sep.)
• Page Start: 1419
• Page End: 1422
• Publication Date: 2014-01-21
• Subjects: Materia medica, Vegetable -- Periodicals; Botany, Medical -- Periodicals; Medicinal plants -- Periodicals; Plant Extracts -- therapeutic use -- Periodicals; Plants, Medicinal -- Periodicals; 581.634
• DOI: 10.1002/ptr.5116
• Languages: English
• ISSNs: 0951-418X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Bacopa monniera</italic> is a traditional Ayurvedic medicinal plant that has been used worldwide for its nootropic action. Chemically standardized extract of <italic>B. monniera</italic> is now available as over the counter herbal remedy to enhance memory in children and adults. Considering the nootropic action of <italic>B. monniera</italic>, we evaluated the effect of clinically available <italic>B. monniera</italic> extract and six of <italic>B. monniera</italic> constituents (bacoside A3, bacopaside I, bacopaside II, bacosaponin C, bacosine, and bacoside A mixture) on recombinant human monoamine oxidase (MAO) enzymes. The effect of <italic>B. monniera</italic> extract and individual constituents on human recombinant MAO‐A and MAO‐B enzymes was evaluated using MAO‐Glo^TM assay kit (Promega Corporation, USA), following the instruction manual. IC₅₀ and mode of inhibition were measured for MAO enzymes. Bacopaside I and bacoside A mixture inhibited the MAO‐A and MAO‐B enzymes. Bacopaside I exhibited mixed mode of inhibition with IC₅₀ and Ki values of 17.08 ± 1.64 and 42.5 ± 3.53 µg/mL, respectively, for MAO‐A enzyme. Bacopaside I is the major constituent of <italic>B. monniera</italic>, which inhibited the MAO‐A enzyme selectively. Copyright © 2014 John Wiley &amp; Sons, Ltd.</p> </abstract>