An Efficient DNA‐Fueled Molecular Machine for the Discrimination of Single‐Base Changes. (25th July 2014)

• Record Type: Journal Article
• Authors: Song, Tingjie; Xiao, Shiyan; Yao, Dongbao; Huang, Fujian; Hu, Maobin; Liang, Haojun
• Is Part Of: Advanced materials. Volume 26:Number 35(2014)
• Page Start: 6181
• Page End: 6185
• Publication Date: 2014-07-25
• Subjects: Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adma.201402314
• Languages: English
• ISSNs: 0935-9648

Abstract

<abstract abstract-type="graphical" xml:lang="en" id="adma201402314-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>A new strategy for single‐base polymorphism (SNP) detection</bold> based on the assembly of DNA‐AuNPs (gold nanoparticles) driven by a DNA‐fueled molecular machine, is established and optimized. It is highly efficient, works at room temperature, and is easy to handle. A single‐base change on an oligonucleotide strand is unambiguously discriminated for either SNPs or insertions and deletions (indels). The strategy is demonstrated to detect a mutation in the breast cancer gene <italic>BRCA1</italic> in homogeneous solution at room temperature. <boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgh1c43bnv8" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>