A Highly Sensitive and Selective Enzymatic Biosensor Based on Direct Electrochemistry of Hemoglobin at Zinc Oxide Nanoparticles Modified Activated Screen Printed Carbon Electrode. Issue 9 (2nd September 2014)

• Record Type: Journal Article
• Authors: Palanisamy, Selvakumar; Karuppiah, Chelladurai; Chen, Shen‐Ming; Periakaruppan, Prakash
• Is Part Of: Electroanalysis. Volume 26:Issue 9(2014:Sep.)
• Page Start: 1984
• Page End: 1993
• Publication Date: 2014-09-02
• Subjects: Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201400304
• Languages: English
• ISSNs: 1040-0397

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>A sensitive enzymatic biosensor has been developed for the detection of hydrogen peroxide (H₂O₂), nitrite (<inline-formula><alternatives><tex-math notation="tex"><![CDATA[${{\rm NO}{{- \hfill \atop 2\hfill}}}$]]></tex-math><inline-graphic xlink:href="ark:/27927/pgh18r9kq43" mimetype="image" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></alternatives></inline-formula>) and trichloroacetic acid (TCA) by using hemoglobin (Hb) immobilized on activated screen printed carbon electrode (ASPCE) and zinc oxide (ZnO) composite. A pair of well defined redox peaks is observed with a heterogeneous electron transfer rate constant (K_s) of 5.27 s⁻¹ for Hb at ASPCE/ZnO. The biosensor exhibits the detection of H₂O₂, TCA and <inline-formula><alternatives><tex-math notation="tex"><![CDATA[${{\rm NO}{{- \hfill \atop 2\hfill}}}$]]></tex-math><inline-graphic xlink:href="ark:/27927/pgh18r9kps2" mimetype="image" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></alternatives></inline-formula> in the concentration range of 0.5–129.5 µmol L⁻¹, 2.5–72.5 mmol L⁻¹ and 0.2–674 µmol L⁻¹ with the detection limit of 0.083 µmol L⁻¹, 0.12 mmol L⁻¹ and 0.069 µmol L⁻¹, respectively.</p> </abstract>