Synergistic effect of smoking with genetic variants in the AMPKα1 gene on the risk of coronary artery disease in type 2 diabetes. Issue 6 (September 2014)

• Record Type: Journal Article
• Authors: Ma, Xiaowei; Zhang, Jianwei; Deng, Ruifen; Ding, Shan; Gu, Nan; Guo, Xiaohui; Weng, Jianping; Pozzilli, Paolo
• Is Part Of: Diabetes/metabolism research and reviews. Volume 30:Issue 6(2014:Sep.)
• Page Start: 483
• Page End: 488
• Publication Date: 2014-09
• Subjects: Diabetes -- Periodicals; Metabolism -- Periodicals; 616.642
• DOI: 10.1002/dmrr.2520
• Languages: English
• ISSNs: 1520-7552

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="dmrr2520-sec-0001" sec-type="section"> <title>Background</title> <p>Increasing evidence suggests that adenosine monophosphate‐activated protein kinase (AMPK) plays a critical physiological role in the cardiovascular system. The objective of this study was to assess the possible correlation between the genetic variability of the <italic>AMPKα1</italic> (<italic>PRKAA1</italic>) gene and the risk of cardiovascular disease, as well as the interactive effects of the genetic variations and environmental factors, on the risk in Chinese patients with type 2 diabetes.</p> </sec> <sec id="dmrr2520-sec-0002" sec-type="section"> <title>Methods</title> <p>Five haplotype‐tagging single nucleotide polymorphisms (SNPs) at the <italic>AMPKα1</italic> locus and 404 unrelated Chinese Han subjects with type 2 diabetes were studied; 260 individuals with coronary artery disease and 144 non‐coronary artery disease controls were genotyped using the polymerase chain reaction–restriction fragment length polymorphism assay.</p> </sec> <sec id="dmrr2520-sec-0003" sec-type="section"> <title>Results</title> <p>Minor allele C at rs3805489 was protective from coronary artery disease in type 2 diabetic subjects compared with allele A (OR 0.67, 95% CI 0.48–0.92, <italic>p</italic> = 0.015). There was no significant correlation between the genotypes at five SNPs and the risk of coronary artery disease. In addition, a significant interaction was identified between smoking status and rs3805489 (<italic>p</italic> = 0.018 for interaction). The smokers with genotype AA at the SNP had a three‐fold higher risk of coronary artery disease compared with non‐smokers with genotypes AC or CC (OR′ 3.02, 95% CI′ 1.39–6.57, <italic>p</italic>′ = 0.005, after adjustment for other known coronary artery disease risk factors).</p> </sec> <sec id="dmrr2520-sec-0004" sec-type="section"> <title>Conclusions</title> <p>The genetic variability at the <italic>AMPKα1</italic> locus has synergetic effects with smoking to increase the risk of coronary artery disease in the Chinese Han population with type 2 diabetes. Copyright © 2014 John Wiley & Sons, Ltd.</p> </sec> </abstract>