Parmelia sulcata Taylor and Usnea filipendula Stirt induce apoptosis‐like cell death and DNA damage in cancer cells. (1st August 2014)

• Record Type: Journal Article
• Authors: Ari, F.; Aztopal, N.; Oran, S.; Bozdemir, S.; Celikler, S.; Ozturk, S.; Ulukaya, E.
• Is Part Of: Cell proliferation. Volume 47:Number 5(2014:Oct.)
• Page Start: 457
• Page End: 464
• Publication Date: 2014-08-01
• Subjects: Cell proliferation -- Periodicals; 571.84
• DOI: 10.1111/cpr.12123
• Languages: English
• ISSNs: 0960-7722

Abstract

<abstract abstract-type="main" id="cpr12123-abs-0001"> <title>Abstract</title> <sec id="cpr12123-sec-0001" sec-type="section"> <title>Objectives</title> <p>Successful cancer treatments still require more compounds to be isolated from natural sources. Thus, we have investigated anti‐proliferative/apoptotic effects of methanolic extracts of lichen species <italic>Parmelia sulcata</italic> Taylor and <italic>Usnea filipendula</italic> Stirt on human lung cancer (A549, PC3), liver cancer (Hep3B) and rat glioma (C6) cells.</p> </sec> <sec id="cpr12123-sec-0002" sec-type="section"> <title>Materials and methods</title> <p>Anti‐proliferative effects were monitored by MTT and adenosine triphosphate viability assays, while genotoxic activity was studied using the comet assay. Additionally, cell death mode and apoptosis assays (fluorescence staining, caspase‐cleaved cytokeratin 18, caspase‐3 activity and PARP cleavage) were performed.</p> </sec> <sec id="cpr12123-sec-0003" sec-type="section"> <title>Results</title> <p>Extracts produced anti‐population growth effects in a dose‐dependent manner (1.56–100 μg/ml) by inducing apoptosis‐like cell death. This resulted in the lines having the presence of pyknotic cell nuclei. In addition, significant increase in genetic damage in the cell lines was seen, indicating that DNA damage may have been responsible for apoptotic cell death.</p> </sec> <sec id="cpr12123-sec-0004" sec-type="section"> <title>Conclusion</title> <p>In this study, methanolic extracts of <italic>Parmelia sulcata</italic> and <italic>Usnea filipendula</italic> induced apoptosis‐like cell death by causing DNA damage, to cancer cells.</p> </sec> </abstract>